FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497339
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.6398050424358436No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.5374603640575141No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT22320.44878845214230134No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.37740856840103026No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.24108304395995486No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.2298231186373882No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.20670005770711727No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.20649898761207144No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.15804109470602545No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7830.15743788442088796No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.14658009928841292No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7170.14416725814786294No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.13592338425098374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.12948914120951704No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.12566880940364622No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.12526666921355453No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA5960.11983777664731701No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.11280032332071284No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.11219711303557534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14200.054.4720882
GTGGTAT15050.050.558581
ATATCAA7450.041.082281
GGTATCA32000.039.851951
GGCATCG450.001662921937.774167
GTATCAA60250.037.746361
AAGTACG2550.036.6742171
CGCGAGA709.237359E-636.4287452
GTCGAGG14350.035.83288
ATGCGAC301.595331E-435.41683220-21
TATCAAC74250.034.6870352
AGTATCA2350.034.368781
GTACTAG500.002784660634.0071
CCGCGAG751.479911E-534.0071
TTAGACT500.002788782633.9967424
TATGCAA500.002788782633.9967429
TCGAGGT15000.033.1468249
TACTAGG652.5512106E-432.6924632
TATACGT1306.7666406E-1032.6891754
GCATCGT652.552717E-432.6891758
Produced by FastQC (version 0.11.5)