FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933225
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.1635189798815934No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.12494307374963165No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.1238715208015216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTG1056.91216E-1140.475915
GTGGTAT7600.040.273671
GTACGAC559.560841E-538.6360933
GGTATCA15000.035.9927251
GTATCAA28100.035.7032661
TGGTATC8950.035.139422
TATACGT1008.063398E-833.9997634
GTCGAGG5650.032.3414428
TATCAAC34200.030.9427682
ATCAACG34700.030.374433
TCAACGC34900.030.322144
CAACGCA36250.029.1929045
ATCGGAT300.00680866828.3316216-17
AACGCAG37850.027.9588576
AGTCGAG8650.025.5462197
CCATTAG850.001224516825.0065291
CGCAGAG42850.024.5946258
ACGCAGA44350.023.8586067
TCGAGGT6950.023.8461999
ACTCCGG1251.7503886E-523.7998356
Produced by FastQC (version 0.11.5)