FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933225
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32550.3487904846098208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG202.5996612E-455.05861
GTGGTAT7950.034.9743271
AGCGCTC401.9019574E-434.3728919
TGGTATC8000.033.6926542
TAAACGG350.00409939931.42832648
ACGGGCG453.7951767E-430.55531716
GGTATCA17650.028.6990991
GCGACGA1152.1827873E-1128.6938923
TATCGGC400.00786650727.5042112
TGCGACG805.1804454E-727.49831422
GGACGGC858.8033994E-725.877996
CGCCGGT651.0880127E-425.3871412
GTATCAA32200.024.7934671
CGACGAG1151.9104846E-823.91157524
ACGGCGC701.8010188E-423.569988
ACGGGAC600.002034068322.9189433
TGGCGAA600.002034702322.91771514
TCGACGG600.002034702322.91771515
GTACTAG1605.4569682E-1122.3675581
AGAGTAC19500.022.00218811
Produced by FastQC (version 0.11.5)