EGAF00005412189 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005412189
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43229
Sequences flagged as poor quality	0
Sequence length	61
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3887	8.99164912443036	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA	463	1.0710402738902125	No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	259	0.5991348400379375	No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA	196	0.45339933840708785	No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA	181	0.41870040944736175	No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	173	0.40019431400217453	No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.3516158134585579	No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA	142	0.32848319415207383	No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118	0.27296490781651206	No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA	106	0.24520576464873117	No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA	100	0.23132619306484073	No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC	98	0.22669966920354392	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG	87	0.20125378796641147	No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.19894052603576304	No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	79	0.1827476925212242	No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG	73	0.16886812093733372	No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.16655485900668532	No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA	65	0.15036202549214647	No Hit
GGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.14342223970020127	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA	62	0.14342223970020127	No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.14110897776955286	No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA	59	0.13648245390825603	No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT	58	0.13416919197760763	No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC	54	0.12491614425501399	No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA	53	0.12260288232436559	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC	53	0.12260288232436559	No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC	51	0.11797635846306877	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATC	49	0.11334983460177196	No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGC	49	0.11334983460177196	No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCT	47	0.10872331074047514	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTCTGCGTTGATAC	45	0.10409678687917834	No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.10409678687917834	No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCT	44	0.10178352494852994	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTATT	15	0.0057484917	54.994205	21
TTTGTAT	15	0.0057484917	54.994205	20
GTGGTAT	100	0.0	52.426746	1
TGGTATC	115	0.0	45.4827	2
AGCGCCG	70	3.8526196E-9	35.394432	2
GCCGCGA	80	3.7471182E-10	34.371376	5
GAGCGCC	75	7.386916E-9	33.111626	1
TGGGACC	95	6.730261E-11	31.83875	4
GGTATCA	270	0.0	31.680878	1
CGGGACC	45	3.7382022E-4	30.552338	17
TACGGGA	65	3.1151394E-6	29.612267	15
CGCCGCG	85	2.586603E-8	29.114578	4
GTATCAA	550	0.0	28.094713	1
GCGCCGC	90	4.456342E-8	27.529003	3
CAGAGTA	170	0.0	27.497103	10
AGAGTAC	170	0.0	27.497103	11
ACGGGAC	60	6.184632E-5	27.497103	16
AGGTTCC	40	0.0077948463	27.497103	32
CCGCGAG	130	8.731149E-11	25.470484	1
TTGGGAC	130	9.094947E-11	25.411388	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content