FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202344
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.7403234096390305No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.5955204997430119No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.5569722848218874No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT7280.35978333926382794No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.2708259202150793No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.23672557624639226No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.2238761712726841No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.21893409243664255No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.20657889534653856No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.19768315344166368No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.16111177005495592No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.16061756217135176No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA2810.13887241529276875No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA2730.13491875222393548No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2400.11860989206499822No Hit
TGGTAGAACCATGCAATGGGTCTTAAAGTTTCTGCTGGATACAGCACATA2330.1151504368797691No Hit
GGCCATACATGCCAAACCTGCAGCTAACATCATTCTCGACAGCGAAAAGT2330.1151504368797691No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2290.11317360534535248No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.11020835804372751No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2220.10971415016012334No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.10921994227651918No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2200.10872573439291505No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2090.10328944767326928No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC2090.10328944767326928No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10230103190606098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA150.001028325884.999993
AAGTACG456.730261E-1175.555561
GTAAAGC302.6402759E-670.833333
ATGTGCA200.003219643463.757
TACATGT200.003219643463.754
CTATAAC302.2507786E-456.6666649
TGGTATC4750.053.684212
TACGTGC651.7862476E-952.3076976
TCTACAT250.00778702551.03
AATGAGT250.00778702551.08
ACGTGCA509.103496E-751.07
ACTGTGC250.00778702551.08
TACTACA250.00778702551.04
ATACCGT250.00778702551.06
ACTGGAC250.00778702551.03
GTGGTAT5100.049.1666681
TATACGT756.339178E-945.3333324
GGTATCA11150.043.834081
GGTACAT505.4359974E-542.52
TACTGGA409.3011785E-442.52
Produced by FastQC (version 0.11.5)