FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202344
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27221.3452338591705215No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.14776815719764363No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.11465622899616494No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA2270.11218518957814415No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC2050.10131261613885265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAG150.00574905755.0668141
CATATCG202.6087582E-454.9987684
TGCCGTC202.6087582E-454.99876849
GTCATAT150.00577726354.99876413
TCTTGGG150.00577726354.9987643
TTCAGGC150.00577726354.99876451
CGTCTAC150.00577726354.99876452
GTCTACG150.00577726354.99876453
GCTGATT150.00577726354.99876433
TCTACGA150.00577726354.99876454
TCTGTCG305.407146E-754.9987648
GCCGTCT150.00577726354.99876450
GAGATGC150.00577726354.99876445
CTGTCGC351.565706E-647.14189
TGGCTCT303.4814137E-545.8323024
AGCCTAC257.7943254E-444.053451
AGGATCC451.794815E-742.7768176
CGCCCAG403.9243623E-641.24907713
CTCTATA358.580777E-539.333441
GGATCCA504.102858E-738.4991347
Produced by FastQC (version 0.11.5)