FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634516
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.31646168102932No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.27390956256422216No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.18880532563402658No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7440.1172547264371584No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.1077987001115811No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.10496189221390793No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.10007627861235967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10050.044.40271
TTATCCG409.312931E-442.5030754
TGGTATC11350.042.3158342
ATACGGG1500.039.666411
GTATCAA38350.039.2305151
TATCAAC48200.036.7713282
TAGTGTA709.247089E-636.4254659
ATATCAA8300.035.843141
GGTATCA19800.035.4164351
AGTATCA2900.035.1721841
TCGAGGT8350.035.1167649
GTCGAGG9200.034.184568
ATCAACG52000.034.0841943
CAACGCA52650.033.501955
TCAACGC52950.033.3121384
AACGCAG55400.031.7622246
TATACGT952.071305E-631.3180544
AAGTACG2800.030.3569471
TAGGATA353.9436188E-430.35694730-31
CTAACTC853.4866818E-530.002173
Produced by FastQC (version 0.11.5)