FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634516
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36920.5818608199005226No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.12623795144645683No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGT150.005781665455.00181639
GTGGTAT11200.036.117721
TGGTATC12600.032.089452
TACGTCA350.004100763231.42465434
GCCCCTA400.00786925127.50090840
GCGGACG400.00786925127.50090813
TTTCCGG400.00787227927.4987427
TTCGAGC606.315342E-527.4987425
GTATCAA45100.026.5420071
CCGACCG550.001225712425.0008249
CAGAGTA31250.024.8168210
AGAGTAC31450.024.57155811
ATTCGAG701.7999437E-423.57034724
GCTCGCA701.800906E-423.56849130
TATCAAC51300.023.5376242
GGTATCA23550.023.3700771
TCGAGGG2850.023.1586579
CGGGGTA2750.023.00075717
GTCATAC600.0020266922.9318891
ACGGGGT4650.022.4738616
Produced by FastQC (version 0.11.5)