FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133445
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12380.9277230319607329No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.20682678256959794No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.1903405897560793No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.11915021169770317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.11690209449585973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGATG150.00576731255.012379
GTTCGTT150.005775877354.99175322
CAGATAG150.005775877354.99175347
AGTCGTC150.005775877354.99175347
ACAGGTA257.808326E-444.026412
ACGTGCA451.7851744E-742.7874037
CGTGCAA403.907622E-641.2592778
TATACGT709.276846E-1139.309294
TACGTGC709.276846E-1139.294556
CGGGTAA358.634194E-539.27982317
GTGGTAT5400.036.74381
GGCCCTA300.001917414436.67491512
GGGCCCT300.001917414436.67491511
GCTCCGA300.001917414436.67491511
ATACGTG751.9463187E-1036.674915
GCAGGTA458.794734E-636.6611715
CGGGAGC300.001920949236.66116717
TGGTATC5800.034.632842
TTATACG803.8744474E-1034.395633
AGGTATA401.8942435E-434.36984617
Produced by FastQC (version 0.11.5)