FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114492
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22231.9416203752227228No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT1680.1467351430667645No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC1630.14236802571358698No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.12926667365405442No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT1340.11703874506515739No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTC1340.11703874506515739No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC1300.11354505118261538No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT1280.11179820424134436No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT1240.10830451035880237No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAG1160.10131712259371835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGG150.00576454655.01399248
TGGTCAG150.005774531554.98994415
GGAATTA257.816943E-444.01119655
AAATACG257.833804E-443.9919624
CCCTATT257.833804E-443.9919633
GCTATAG257.833804E-443.9919614
AGGGTAA257.833804E-443.9919619
GGCCCTA257.833804E-443.9919631
GCACCAT257.833804E-443.991966
GTGCTAT257.833804E-443.9919612
GCCCTAT257.833804E-443.9919632
TAGGGTA257.833804E-443.9919618
TGCTATA257.833804E-443.9919613
ATACGGG257.833804E-443.9919626
AATACGG257.833804E-443.9919625
GTGGTAT3750.037.442261
TGGTATC3850.037.136072
ATAGGGT300.001920115936.65996617
CTATAGG300.001920115936.65996615
GTATAAA401.8783717E-434.413841
Produced by FastQC (version 0.11.5)