FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150047
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.7224403020386946No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.635800782421508No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.45852299612787994No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4130.2752470892453698No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.25991855885155984No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.25525335394909593No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.23659253433924035No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.22392983531826696No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.17994361766646452No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA2590.1726125813911641No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1632821715862363No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.1626157137430272No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.13595740001466206No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.13262511079861644No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.12995927942577992No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.1272934480529434No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.12662699020973428No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1650.1099655441295061No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT1570.10463388138383306No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.10330096569741481No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA1550.10330096569741481No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10196805001099656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG800.074.393681
GTGGTAT5000.055.2638661
TGGTATC5650.053.4204862
AGTAAGC250.007775398451.0128023
GCTGTGC250.007785657450.99589
ATATCAA2800.050.1018641
GTACATG655.520087E-639.2406161
AGTATCA1750.038.8668981
CAGTTCT559.5080584E-538.646064
GAGACTC450.001656394737.7872665
GACAGGT601.5865876E-435.425561
TGTCGTT301.5934955E-435.4137584-85
GGTATCA11300.035.3628581
ACGTGCA500.00277787734.0085337
GCAAGTG250.002786843833.997228-29
TGGACAT250.002786843833.997228-29
GTATCAA21050.033.5238531
TATCAAC25850.033.219172
GTCGAGG4100.033.1688
ATATAAG1700.032.5081561
Produced by FastQC (version 0.11.5)