FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516303
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAG177490.5047630991982204No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCACTGAGGAGC163220.46418070342629747No Hit
GTCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGAAGTGGGACCAAGG150640.4284044918768377No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC144620.411284238019306No Hit
CCATAACCTGACCCGATGTTGGAGCTGCTTCCAGTGCAGGAGATGGTGAC134370.38213430412566834No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGGAACA134360.3821058651657721No Hit
GCCTTGGGCTGACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATA129440.36811389689682605No Hit
GTCTTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC118650.33742825916879177No Hit
GGTTATGGTGTCCACTGGTACCAGCACTTTCCAGGTACAGCCCCCAAACT115350.32804340240303526No Hit
CCACTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT113940.32403350905766654No Hit
GTCTGGGGCCCCGGGGCAGAGGGTCACCATCTCCTGCACTGGAAGCAGCT109400.31112222126477723No Hit
CCTCAGTGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA108710.30915993303193723No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGG108050.3072829616787859No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAGTGGC104250.2964761569182178No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT103540.29445699076558535No Hit
GTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA98560.28029438873726187No Hit
CTCCTGCACTGGAAGCAGCTCCAACATCGGGTCAGGTTATGGTGTCCACT88400.2514004054826902No Hit
TTCCAGGTACAGCCCCCAAACTCCTCATCTTTGATAACATCAATCGGCCC85370.24278340063413192No Hit
GACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGCACTCAGG85130.24210086559662236No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCA83110.2363561956975835No Hit
GTACCTGGAAAGTGCTGGTACCAGTGGACACCATAACCTGACCCGATGTT83070.2362424398579986No Hit
TCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT79110.22498061173909073No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC77350.21997535479735394No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCT76410.21730209256710814No Hit
GATTATTACTGCCAGTCCTATGACAGCACCCTGAGTGCCTATGTCTTCGG74680.21238215250505998No Hit
CCTGAGGGCCGATTGATGTTATCAAAGATGAGGAGTTTGGGGGCTGTACC74330.2113867889086919No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT73370.2086566487586536No Hit
GTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC73280.20840069811958753No Hit
GCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG71890.2044476826940113No Hit
GGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGT70330.2000112049501991No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAAGACGGTGACCTTGGTC69670.19813423359704782No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT68110.19369775585323562No Hit
CTGTCACTCTGTTCCCGCCCTCCACTGAGGAGCTCCAAGCCAACAAGGCC63550.18072959014055387No Hit
GTTCCCGCCCTCCACTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT63410.18033144470200663No Hit
GTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCT62530.1778288162311382No Hit
TTCCAGGCCACTGTCACAACTCCCGGGTAGAAGTCACTGATCAGACACAC61470.17481428648213762No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAGTGGCCTG60730.17270980344981648No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGCTGGG60610.1723685359310617No Hit
AGGTTATGGTGTCCACTGGTACCAGCACTTTCCAGGTACAGCCCCCAAAC60370.1716860008935521No Hit
GGTTAGAGCTGGGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTCC60070.17083283209666517No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT59980.17057688145759908No Hit
CCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG59340.1687567880242402No Hit
CATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGA59230.16844395946538168No Hit
GGGTTAGAGCTGGGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTC59200.168358642585693No Hit
CCCCTACAGAGTGTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGC58970.16770454650807964No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC57930.16474689467887155No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGGAACAGAGT57890.1646331388392866No Hit
TCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGAAGTGGGACCAAGGT57620.16386528692208835No Hit
CCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT57290.16292680124551268No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGGGAGC56550.1608223182131915No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC56040.1593719312584837No Hit
ACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGCACTCAGGG55440.15766559366470978No Hit
GCCCAGTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAAT54540.15510608727404893No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.15320067696100137No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCACCCTGAGTGCCT53660.1526034588031805No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA53480.15209155752504833No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAG53380.151807167926086No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG53230.15138058352764253No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT51990.14785415250050976No Hit
GCTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGG51660.14691566682393412No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC49930.141995726761886No Hit
GTCACAACTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT49170.13983436580977238No Hit
GTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG48650.1383555398951683No Hit
CCTCAGGCTCCCTGGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGAT48360.13753081005817758No Hit
GTGCAGGAGATGGTGACCCTCTGCCCCGGGGCCCCAGACAGTGAGGGCGG48290.13733173733890394No Hit
GTGCTGGTACCAGTGGACACCATAACCTGACCCGATGTTGGAGCTGCTTC47680.1355969607852338No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCA47520.1351419374268941No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC47410.13482910886803556No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGC45910.13056326488360076No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT45030.12806063641273235No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGG44960.12786156369345872No Hit
GTGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAA44920.1277478078538738No Hit
GTTTGGGGGCTGTACCTGGAAAGTGCTGGTACCAGTGGACACCATAACCT44730.1272074676158454No Hit
GCCCCAGACAGTGAGGGCGGCTGCGTCAGGACAGACTGGGCCCAGGACCC44720.12717902865594918No Hit
CGCATGAGGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAGTGTTCA44710.12715058969605292No Hit
GCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGGGAGCCTGAGGTGCC44430.12635429881895843No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC44090.12538737418248655No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAGGGGAGCA43120.12262879507255206No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC43090.12254347819286336No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG42900.12200313795483494No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT42720.12149123667670278No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGAAGCAGCTCCAACATCGGGTC42710.12146279771680654No Hit
GTCAGGGACTCCTGAGGGCCGATTGATGTTATCAAAGATGAGGAGTTTGG42710.12146279771680654No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT42590.12112153019805177No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC42570.1210646522782593No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC42260.1201830445214761No Hit
GGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAGTGTTCATAGGTTC42030.11952894844386276No Hit
CCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGC41880.1191023640454193No Hit
CTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGT41750.11873265756676829No Hit
CTGTCACAACTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC41110.11691256413340943No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCACTGGGCTCCGGGCTGAGGATG41070.11679880829382451No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA40080.11398335126409755No Hit
GTACCAGCACTTTCCAGGTACAGCCCCCAAACTCCTCATCTTTGATAACA39970.11367052270523899No Hit
CTTCTACCCGGGAGTTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG39820.11324393830679552No Hit
CTCCTGAGGGCCGATTGATGTTATCAAAGATGAGGAGTTTGGGGGCTGTA39440.1121632578307387No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC39370.11196418511146508No Hit
CCCGTGGGTGGGGTTAGAGCTGGGAACCTATGAACACTCTGTAGGGGCCA39110.11122477215416304No Hit
GCCCCCAAACTCCTCATCTTTGATAACATCAATCGGCCCTCAGGAGTCCC39070.11111101631457812No Hit
GACATAGGCACTCAGGGTGCTGTCATAGGACTGGCAGTAATAATCAGCCT38850.11048535919686102No Hit
TCCGAAGACATAGGCACTCAGGGTGCTGTCATAGGACTGGCAGTAATAAT38850.11048535919686102No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACTCCTGAGGGCCGATT38820.11040004231717233No Hit
CCTACAGAGTGTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGCCT38240.10875058264319087No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38100.10835243720464362No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA37580.10687361129003957No Hit
CAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCC37500.1066460996108697No Hit
CATGAGGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAGTGTTCATA37340.10619107625252999No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC37260.10596356457336015No Hit
GCCTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCTTGGGTCAGCCCAA37040.10533790745564303No Hit
ATGTTGGAGCTGCTTCCAGTGCAGGAGATGGTGACCCTCTGCCCCGGGGC36950.10508195681657696No Hit
GCCCGGAGCCCAGTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCC36920.10499663993688826No Hit
GAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT36350.10337561922280304No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT36190.10292059586446334No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG35780.10175459850871782No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG35740.10164084266913288No Hit
CTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCAT35580.10118581931079318No Hit
GCTCCAACATCGGGTCAGGTTATGGTGTCCACTGGTACCAGCACTTTCCA35510.10098674659151956No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGG35510.10098674659151956No Hit
GGAGAAGACAGTGGCCCCTACAGAGTGTTCATAGGTTCCCAGCTCTAACC35480.10090142971183086No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT35370.1005886011529723No Hit
GTGTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGCCTGGAGCTGC35200.10010513883473637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)