FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516303
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTG135090.38418190923819706No Hit
GTCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCTTG134470.38241869372463067No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGG129030.3669478995410805No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCACTGAGGAGCTCCAAGCCAAC120340.34223444339125497No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGC115940.3297213010369129No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGGAACAGAGTGACAGTG113830.323720680498808No Hit
GCCTTGGGCTGACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGCACTCAGGG106950.3041546760902004No Hit
GGTTATGGTGTCCACTGGTACCAGCACTTTCCAGGTACAGCCCCCAAACTCCTCATCTTTG106880.3039556033709268No Hit
GTCTGGGGCCCCGGGGCAGAGGGTCACCATCTCCTGCACTGGAAGCAGCTCCAACATCGGG105000.2986090789104352No Hit
GTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCG101030.2873188118316311No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCT95050.2703123138136844No Hit
GTCTTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCACTGAGGAGC90610.25768541561975744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89560.2546993248306531No Hit
GTCCTGGGCCCAGTCTGTCCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCGGGGCAGAGG89500.25452869107127574No Hit
CCACTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCC88580.2519123067608224No Hit
CCTCAGTGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAAGACGGT88440.25151416132227516No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAGTGGCCTGGAAGGCAG79820.2269997778917232No Hit
CTCCTGCACTGGAAGCAGCTCCAACATCGGGTCAGGTTATGGTGTCCACTGGTACCAGCAC76720.21818370032389134No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG71270.20268446718044492No Hit
TTCCAGGTACAGCCCCCAAACTCCTCATCTTTGATAACATCAATCGGCCCTCAGGAGTCCC69870.19870301279497243No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAG69800.19850394007569885No Hit
GATTATTACTGCCAGTCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGAAGTGGGACCA67610.19227580785842405No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT66820.19002913002662172No Hit
GTCCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCGGGGCAGAGGGTCACCATCTCCTGCA66570.18931815602921592No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTG64960.18473948348592256No Hit
GCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCC64910.1845972886864414No Hit
GACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGCACTCAGGGTGCTGTCATA64750.1841422653281017No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCC64680.18394319260882808No Hit
TCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCACCCT64440.1832606575713185No Hit
GGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGC63710.1811846134988936No Hit
GGGTTAGAGCTGGGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCC62190.17686189159466634No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAAGACGGTGACCTTGGTCCCACTTCCGAA61740.1755821383993359No Hit
GTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC61190.17401799560504314No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG61140.17387580080556198No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCAC59470.16912649450289124No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC56080.15948568709806862No Hit
GTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACTCCTG55660.1582912507824269No Hit
AGGTTATGGTGTCCACTGGTACCAGCACTTTCCAGGTACAGCCCCCAAACTCCTCATCTTT53000.1507264874500292No Hit
TTCCAGGCCACTGTCACAACTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCT52460.14919078361563268No Hit
GGTTAGAGCTGGGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCC52060.1480532252197834No Hit
CCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCT51760.14720005642289644No Hit
CCATAACCTGACCCGATGTTGGAGCTGCTTCCAGTGCAGGAGATGGTGACCCTCTGCCCCG51310.145920303227566No Hit
CCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG51130.14540840194943383No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAGTGGCCTGGAAGGCAGATG49850.14176821508271611No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAA49820.14168289820302743No Hit
GTTCCCGCCCTCCACTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49730.14142694756396135No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCG49630.14114255796499903No Hit
CTGTCACTCTGTTCCCGCCCTCCACTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTG49570.14097192420562166No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGCTGGGAACCTATGAAC49310.14023251124831962No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGA49290.14017563332852714No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCA49280.14014719436863093No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGGAACAGAGTGACAGTGGGGT49230.14000499956914975No Hit
CATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAG48840.13889588013319673No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA48270.1372748594191115No Hit
TCCTATGACAGCACCCTGAGTGCCTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCTTGG47730.13573915558471497No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAGGGGAGCACCGTGGAGAAG47610.1353978880659602No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGG46650.13266774791592192No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA46630.13261086999612945No Hit
GTCACAACTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGA46560.13241179727685584No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCG45030.12806063641273235No Hit
GGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAGTGTTCATAGGTTCCCAGCTCTAAC44880.12763405201428887No Hit
CCCCTACAGAGTGTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGCCTGGAGCTGCA44790.12737810137522276No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCT44720.12717902865594918No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAACTCCCGGG44500.12655337153823207No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGAC43980.125074545623628No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCCATCACTGGGC43880.12479015602466569No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCC43660.12416449890694857No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTC43490.12368103658871263No Hit
CCTGAGGGCCGATTGATGTTATCAAAGATGAGGAGTTTGGGGGCTGTACCTGGAAAGTGCT43350.1232828911501654No Hit
GCACAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCGCCCTCACTGTCTGGGGCCCCGGG42890.12197469899493874No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGTTGTGACAGTGGCCTGGAAG42860.12188938211525001No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACC42810.12174718731576886No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGGGAGCCTGAGGTGCCA42750.12157655355639148No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGC42150.11987021596261756No Hit
ACCCAAGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGCACTCAGGGTGCTGTCATAG41410.1177657329302964No Hit
CCTCAGGCTCCCTGGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCA41210.11719695373237175No Hit
GTTCATAGGTTCCCAGCTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGG40580.11540529925890915No Hit
CTGTCACAACTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTG40200.11432461878285233No Hit
GTGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAAGACGGTGACCT40150.11418242398337118No Hit
GTACCTGGAAAGTGCTGGTACCAGTGGACACCATAACCTGACCCGATGTTGGAGCTGCTTC39570.11253296430938972No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGAAGCAGCTCCAACATCGGGTCAGGTTATGGTG39560.1125045253494935No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCT39110.11122477215416304No Hit
GCTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCCC39110.11122477215416304No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGTGGAGGGCGGGAACAGA39090.1111678942343706No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCC39020.11096882151509697No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGC38840.11045692023696478No Hit
GCCCAGTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGAC38490.10946155664059667No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGATTAT38440.10931936184111551No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTG38210.10866526576350218No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCT37990.10803960864578509No Hit
CTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCAC37710.10724331776869057No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCA37390.10633327105201117No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGG36920.10499663993688826No Hit
GGGTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGTGAT36730.10445629969885986No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC36000.10238025562643491No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCT35890.10206742706757638No Hit
GTACCAGCACTTTCCAGGTACAGCCCCCAAACTCCTCATCTTTGATAACATCAATCGGCCC35600.10124269723058565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)