FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384059
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAAC152910.45185382406157815No Hit
GTATATTATTGTGCAGCATGGGATGACAGCCAGACCTGGGTGTTCGGCGGAGGGACCAGGC150860.4457960100577443No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGTCTGGCTGT136000.4018842461080022No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAC119600.35342173407733135No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTG113770.3361939020566722No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGG98210.2902136162519625No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGACG97700.2887065503290575No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGT96930.28643117628859305No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACCC91880.2715082686206121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91710.2710059133129771No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC88830.2624954233953959No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCG80730.23855967050219867No Hit
GGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGC79310.2343635261678357No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGC78520.23202905150294367No Hit
CTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA76360.22564618406475775No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCT76190.22514382875712274No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGG75970.22449372188841862No Hit
GTCCCAGACCGATTCTCTGACTCCAGGTCTGGCACCTCCGCCTCCCTGGCCATCAGTGGGC74270.21947016881206857No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC74100.2189678135044336No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT73550.2173425463326733No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGACGGTCACCATCTCTTGTT73340.21672198977618298No Hit
ATATTATTGTGCAGCATGGGATGACAGCCAGACCTGGGTGTTCGGCGGAGGGACCAGGCTG68820.20336524865553468No Hit
GCATGGGATGACAGCCAGACCTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTGGGTC67650.19990786212651732No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGAC64930.19187017720435726No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCC64720.19124962064786696No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTG63470.187555831621139No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT63360.18723077818678693No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62580.1849258538341087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG58750.1736080842562142No Hit
CCATAGGAATGATCAGCGGCCCTCAGGGGTCCCAGACCGATTCTCTGACTCCAGGTCTGGC58280.1722192195821645No Hit
TTCCTATGGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAATATACATAAT56730.16763892118902182No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG54900.1622312140538921No Hit
GTCTGGGACCCCTGAGGGCCGCTGATCATTCCTATGGATGAGGAGTTTGGGGGCCGTTCCT54460.16093100031648386No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC54320.1605172959454903No Hit
ATAATATACACCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGAGGCGGAGGTGCCA52980.15655755410883795No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCCTGGTC52970.1565280037966241No Hit
GGAATGATCAGCGGCCCTCAGGGGTCCCAGACCGATTCTCTGACTCCAGGTCTGGCACCTC52400.15484363600043616No Hit
ACCCAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGTCTGGCTGTCATCCCATGCT51570.1523909600866888No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTGGAGAAGACAGGACTCAGGACAATC50160.14822436606453965No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCT49890.14742650763476642No Hit
CTGGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATA49450.14612629389735818No Hit
TTATTGTGCAGCATGGGATGACAGCCAGACCTGGGTGTTCGGCGGAGGGACCAGGCTGACC49280.14562393858972317No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCA49110.14512158328208818No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCT48500.1433190142370449No Hit
GACCCAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGTCTGGCTGTCATCCCATGC47770.14116184144543578No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCCATAGGAATGATCAGCGGCCCTCAGGGGTCC47300.13977297677138606No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGAC45640.13486762494389135No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACC45230.1336560621431246No Hit
GTCTGAAGATGAGGGTGTATATTATTGTGCAGCATGGGATGACAGCCAGACCTGGGTGTTC45070.1331832571477034No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAG44790.13235584840571635No Hit
CTCCAGGTCTGGCACCTCCGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATGAGGGT44640.131912593722509No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG44540.13161709060037075No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCAC44210.13064193029731455No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT44140.1304350781118178No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAA43940.12984407186754132No Hit
GCCCTCAGGGGTCCCAGACCGATTCTCTGACTCCAGGTCTGGCACCTCCGCCTCCCTGGCC43340.1280710531347119No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT43320.12801195251028424No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCAC43240.12777555001257365No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAT43160.12753914751486306No Hit
CTCTTGTTCTGGAAGCGGCTCCAATATCGGAAGTAATTATGTATATTGGTACCAGCAAGTC43120.12742094626600778No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCA42940.12688904064615894No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCAC42820.12653443689959307No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA42590.12585477971867512No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCAT41750.12337255349271392No Hit
GATCATTCCTATGGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAATATAC41580.12287019818507892No Hit
GGCCATCAGTGGGCTCCAGTCTGAAGATGAGGGTGTATATTATTGTGCAGCATGGGATGAC41250.12189503788202274No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGG40710.12029932102247626No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCC40480.11961966384155832No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGC40150.11864450353850214No Hit
CTCCTCATCCATAGGAATGATCAGCGGCCCTCAGGGGTCCCAGACCGATTCTCTGACTCCA39840.1177284438598736No Hit
AGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGA39820.11766934323544595No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTT38950.1150984660728433No Hit
CACTGGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGA38880.11489161388734653No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG38610.11409375545757329No Hit
ACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACC38280.11311859515451711No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGC38170.11279354172016505No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA38050.11243893797359915No Hit
CTTCAGCTGTGGGTGGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTC37920.11205478391481945No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGT37820.11175928079268121No Hit
TACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAG37560.11099097267512181No Hit
CTGATGGCCAGGGAGGCGGAGGTGCCAGACCTGGAGTCAGAGAATCGGTCTGGGACCCCTG36970.10924750425450619No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACC36330.10735628427282148No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCTTGTTGGCTTG35630.10528776241785383No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAATATACATAATTACTTCCGATATTGGA35460.10478540711021882No Hit
GCTGTGGGTGGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC35410.10463765554914971No Hit
GGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG34820.1028941871285341No Hit
GTATATTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCCATAGGAATGATC34740.10265778463082352No Hit
GAACAAGAGATGGTGACCGTCTGTCCGGGGGTCCCAGACGCTGAGGGTGGCTGAGTCAGCA34540.10206677838654704No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC34380.10159397339112586No Hit
CCTCAGGGGTCCCAGACCGATTCTCTGACTCCAGGTCTGGCACCTCCGCCTCCCTGGCCAT34080.10070746402471116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)