FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650183
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT285950.6149220364015782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC263780.5672464933100483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC223790.4812498776929854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC194170.41755345972405816No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA188130.40456472358184614No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG174800.37589918504282516No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG170970.3676629500387404No Hit
GAGTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA165810.35656661253976457No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT161670.34766373710453974No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG150540.3237291951736093No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG149770.3220733463607776No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG149470.3214282104596744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC144500.31074045903139724No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG138960.29882694939102394No Hit
CTACTACTGTCAACAGAGTTACAGTACCCCCTGGACGTTCGGCCAAGGGA137720.29616038766646385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT136250.29299922175105797No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATT136140.29276267192065347No Hit
GTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGGTGAGAGTGA131750.28332218323450925No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT130350.2803115490293608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT127180.27349461300770317No Hit
GTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC121120.26046286780541755No Hit
CTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACT118790.255452312306849No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG117830.2533878774233186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA115720.24885042158555912No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT114930.24715156371265387No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG114140.24545270583974868No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC112830.24263561240493117No Hit
GACTACTACTGTCAACAGAGTTACAGTACCCCCTGGACGTTCGGCCAAGG109470.2354100903125748No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGAT107110.2303350212238959No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC105700.22730288248871064No Hit
CAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGGTGAGAGT104990.22577606085609964No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCC101390.2180344300428607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA99060.21302387454429214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG98250.21128200761131335No Hit
GTCTTCACGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATC97200.20902403195745198No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT95400.20515321655083255No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC94980.20425002628928798No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT94360.2029167454270079No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC92330.19855132582954263No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA90500.19461599683281283No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT90300.19418590623207732No Hit
GTCCAGGGGGTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTC88160.1895839368042075No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA85720.18433683147523441No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA85130.1830680642030647No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT83300.17913273520633488No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA82230.17683175049239996No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACGTGAAGACTTTGCAGACTA79980.17199323123412563No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA79780.17156314063339012No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC79620.17121906815280175No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG78530.16887507437879326No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT78120.1679933886472855No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA76480.1644666457212544No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGATACCTGCTA76230.16392903247033505No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT76080.16360646451978342No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC75360.16205813835713562No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT74860.16098291185529687No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT74810.16087538920511302No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC74770.1607893710849659No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC74600.16042379407434074No Hit
CACCATCAGCAGTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAAC73410.1578647549999645No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT72060.1549616434449999No Hit
GTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAG71600.15397243506330827No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG71130.15296172215157983No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC70030.1505962238475346No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC69240.14889736597462938No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC68620.14756408511234934No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA68560.1474350579321287No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC68360.1470049673313932No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA68200.1466608948508048No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG67510.14517708227826734No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT66680.143392206285215No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC66650.14332769269510467No Hit
ACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGA66140.14223096166322918No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA65480.14081166268080203No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT65320.14046759020021363No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT64950.13967192258885294No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGG64360.13840315531668324No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63650.13687633368407223No Hit
AGTCAGAGCATTAGCAGGTATCTAAATTGGTTTCAGCAGAAACCAGGGAA63370.13627420684304253No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC63300.1361236751327851No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC62410.13420977195951214No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC62380.1341452583694018No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC61220.1316507328851359No Hit
GTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA60730.13059701091333395No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC60630.1303819656129662No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCC60540.13018842484263524No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT60490.13008090219245133No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG60090.12922072099098036No Hit
GTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAACAGAGTTACAGT59820.12864009867998744No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGG59760.12851107149976676No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC58380.12554344635469183No Hit
CGTGAAGACTTTGCAGACTACTACTGTCAACAGAGTTACAGTACCCCCTG58250.12526388746421377No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA58230.12522087840414023No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT57950.12461875156311053No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA57850.12440370626274277No Hit
ATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAAC57390.12341449788105113No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC57260.12313493899057307No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT56760.12205971248873432No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC56630.12178015359825625No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC56380.12124254034733686No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG56310.12109200863707945No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT56070.12057589991619684No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC55840.12008129572535103No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA55630.11962970059457875No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG55450.11924261905391681No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT55310.11894155563340195No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT54850.11795234725171032No Hit
CCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGT54020.11616747125865799No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT53690.11545782176744443No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGC53540.11513525381689281No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACGTGAAGACT53520.11509224475681926No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT53470.11498472210663536No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG53060.1141030363751276No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA52830.1136084321842818No Hit
GCATTAGCAGGTATCTAAATTGGTTTCAGCAGAAACCAGGGAAAGCCCCT51990.11180205166119268No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT51990.11180205166119268No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA51940.11169452901100882No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG51530.11081284327950104No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC51390.11051177985898619No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG51300.11031823908865522No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA51230.1101677073783978No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA51190.1100816892582507No Hit
CCATCAGCAGTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAACAG50320.10821079514505129No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG50140.10782371360438933No Hit
CCCCTAACCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA49920.10735061394358028No Hit
GAGCATTAGCAGGTATCTAAATTGGTTTCAGCAGAAACCAGGGAAAGCCC49560.10657645086225638No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49340.10610335120144734No Hit
GGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATC48820.10498511563953505No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATCTAAATTGGT48590.10449051144868922No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC48570.10444750238861568No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT47520.1021895267347543No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT47400.10193147237431302No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC47300.10171642707394526No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG46720.10046916433181233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21600.058.4477161
TGGTATC22800.057.413062
AAGTACG4150.044.0438461
GGTATCA50250.041.6192171
TATCAAC126500.037.6961632
GTATCAA123550.036.985421
ATCAACG140300.034.1700973
CAACGCA140450.034.0424585
AACGCAG141650.033.6940576
TCAACGC142800.033.571524
CGCAGAG159100.030.0236118
ACGCAGA160800.029.7323067
GATTCAC10100.029.4605981
ATACGGG3650.029.114681
GCAGAGT167350.028.629
GTGCTAG18450.028.338291
GACAGGT8950.027.5467151
GTCGAGG18000.026.6791978
GCTAGAG19500.026.15423
GAATACG19750.025.8217459
Produced by FastQC (version 0.11.5)