FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650183
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTG255800.5500858783407019No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC197960.42570367660799585No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGA197390.4244779183958997No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTG193130.4153169886002336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165830.3566096215998381No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACA159880.34381442622795705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCAC153420.32992249982420047No Hit
GAGTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTG149920.32239591431132925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG144430.3105899273211398No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCT140830.3028482965079009No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATCTAAATT137660.29603136048624323No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCT133900.2879456571924158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC129730.2789782681670807No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAG129280.27801056431542587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC123100.264720764752699No Hit
GTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCA122700.263860583551228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCA122660.2637745654310809No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGATACCTGC119560.25710816111968066No Hit
GTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGGGTACT117860.2534523910134289No Hit
CTACTACTGTCAACAGAGTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAA115520.2484203309848236No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC114310.24581828285037385No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTA108590.2335176916693386No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGT107000.2300984713934914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG106880.2298404170330501No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCA105600.2270878371883429No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGG105440.22674376470775448No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCC104430.22457180717404024No Hit
CTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGGTGA103910.22345357161212798No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGC101640.21857204329378005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT98390.2115830710318282No Hit
CAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCC94170.2025081593563092No Hit
GTCTTCACGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTG93760.2016264736248014No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGATACCTGCTAATG93480.20102434678377173No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC93290.200615760713073No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA92490.19889539831013103No Hit
GACTACTACTGTCAACAGAGTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGG91760.19732556761744646No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCC89980.19349776127090051No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAAC88350.18999252287490623No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTG86410.18582064404777188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACT85260.18334762309354277No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG83440.17943379862684974No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC81980.1762941372414806No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGT80950.1740791706476928No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG79470.1708965002022501No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT78740.16932666950956554No Hit
GTCCAGGGGGTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGAC78060.16786436146706485No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACA77130.1658644401736448No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC74950.16117645262562785No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGG74710.16066034390474526No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGT72660.15625191524720639No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA72010.15485412079481603No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGAC70290.15115534162849076No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG69370.14917692486510745No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGATACCTGCTAATGCTCTGACT68950.1482737346035629No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCA68030.14629531784017963No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT67650.14547814569878217No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAAC66760.14356424252550923No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTG64620.1389622730976394No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTT63520.13659677479359417No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCAT63270.13605916154267478No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGG63000.13547853923168185No Hit
GTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGATGG62810.13506995316098314No Hit
CACCATCAGCAGTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAACAGAGTTACAGT62590.13459685350017409No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTG62160.13367215870859275No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTG61980.1332850771679308No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGA61970.13326357263789404No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG61670.13261843673679077No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAAC60760.13066152450344426No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGG60360.12980134330197327No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAG60050.12913470287083326No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAG59890.12879063039024485No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACT59290.12750035858803838No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCT58970.12681221362686157No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT58410.12560795994480217No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTC58370.1255219418246551No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTG58060.12485530139351504No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCC57910.12453273344296342No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCC57900.12451122891292665No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTC57360.12334998429094082No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAG56790.12212422607884464No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC56380.12124254034733686No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATGGAT56320.12111351316711622No Hit
ACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGC55100.11848996050262968No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACGTGAA54890.11803836537185741No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA54520.11724269776049673No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAG54490.11717818417038642No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA53570.11519976740700312No Hit
AGTCAGAGCATTAGCAGGTATCTAAATTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAACC53090.11416754996523794No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCATCT53080.11414604543520115No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCA52770.11347940500406113No Hit
GTTACAGTACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGC52280.11242568303225917No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTAC52120.11208161055167075No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA51830.1114579791806043No Hit
GTCTGCAACGTGAAGACTTTGCAGACTACTACTGTCAACAGAGTTACAGTACCCCCTGGAC51520.11079133874946426No Hit
ATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAGATA51270.1102537254985449No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGA51160.11001717566814037No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCA51090.10986664395788294No Hit
CGTGAAGACTTTGCAGACTACTACTGTCAACAGAGTTACAGTACCCCCTGGACGTTCGGCC50970.10960858959744166No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA50700.10902796728644872No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT50420.10842584044541903No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATCTAAA50360.10829681326519837No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCT49770.10702804599302866No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAGTCTGCAAAGTCTTCACGTTGCAGACTGCTGAT49320.10606034214137379No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGT48280.10382387101754921No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTT47940.10309271699629886No Hit
CCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT47920.10304970793622531No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAG47530.1022110312647911No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47380.10188846331423947No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTC47320.10175943613401882No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCT47130.10135085006332009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG202.61476E-454.9987250
GTGGTAT24250.041.4264371
TGGTATC24950.040.2377472
TCGTCAA401.901973E-434.37641554
GACCGAC606.320063E-527.50113754
CGTAACC400.00787976127.49699231
GGTATCA52850.026.7679181
GTATCAA133300.026.67651
AGAGTAC85750.026.0084211
TATCAAC137250.025.5911542
ATCAACG150050.023.4259323
TCAACGC152000.023.0867254
CAACGCA152350.022.9048635
AACGCAG154550.022.7903676
TACGCCC2000.022.00043542
GTACCGT752.8772344E-421.9968876
AGCCTGA27400.021.475066
GAGTACG104050.021.3548512
AGTACGG103800.021.35306713
GACCCGT1559.0221874E-1021.291236
Produced by FastQC (version 0.11.5)