FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464198
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95082.04826388739288No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC18990.4090926716616616No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14330.3087044752454771No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA10880.234382741847229No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10780.23222848870525079No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC7740.16673919318911326No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA6920.14907431742489197No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAAT6890.1484280414822985No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.13679507451561618No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5870.1264546594341208No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5210.11223658869706461No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5210.11223658869706461No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC5150.1109440368118777No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.10642010521372344No Hit
GTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.10598925458532782No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC4850.10448127738594308No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTT4680.10081904704458011No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4650.10017277110198665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGAT150.005750395455.0748981
TATCACG202.606972E-455.0214542
AGGCGGC202.6097507E-455.00959454
ACGGACA150.00577992155.0036663
TACTGGG150.00578485354.99181415
GTACTGG251.1882221E-554.98588614
AGTCTGG150.005792256454.974048
TGCAGAG150.005792256454.974048
GCGGTAT257.7991694E-444.059921
AAGGCAG257.869902E-443.979235
CCTTAGG403.8981434E-641.306171
CTTAGGA403.9242495E-641.266092
ATGCGAC651.9736035E-938.07535621
CTTAGTT300.00192177636.67701738
TGCTTAA300.001924829936.66515719
TCACGCA300.001925848736.661214
CCAGAGT300.00192686836.6572579
CAAAGTA300.00192686836.65725710
CACGCAG300.001928907736.649365
GACCGTT458.861476E-636.6493577
Produced by FastQC (version 0.11.5)