FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808090
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATAGGTGCTA208700.5480437699739239No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC200010.5252239311570893No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC188640.49536644354518933No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCACTGATGTT172630.45332436995974357No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC158710.4167706120391062No Hit
GATTATTACTGCTGCTCATATTCACCTACTAGCACCTATGTGGTATTCGG157130.41262155043604537No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT156120.4099693021961141No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC151890.398861371448679No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG144110.37843118203613885No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC141360.3712097140561279No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA140290.3683999065148145No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG136140.35750205483588887No Hit
GTTCCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA133270.34996546825311375No Hit
GTGTTGGAACCAGGAGACAACATTATAACTCCCAACATCAGTGCTGGTTC133020.3493089711640219No Hit
CTCCTGCACTGGAACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCCT127690.3353124532245824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA111800.2935854982419008No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAG111540.2929027412692452No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA110740.2908019505841511No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC108550.285051036083706No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA107650.2826876465629751No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA104700.2749409809116906No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCACTGATGTTGGGAGTTATA103450.2716584954662311No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT102130.26819219083582585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA100910.2649884850410573No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT99830.2621524176161803No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC99140.2603404856502866No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT93170.2446633351627719No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG93100.24447951597782613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT92170.2420373468064043No Hit
CCTATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC88910.23347662476464578No Hit
CCCCTGAGGGCCGCTGAGTGACCTCATAAATCATGAGTTTGGGGGCTTTG88300.23187477186726152No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC87880.23077185675758716No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA83990.22055676205131708No Hit
GGACACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT83200.21848223124978663No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG82730.21724801672229388No Hit
CCACATAGGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCCTCG81840.21491088708512668No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG81660.21443820918098047No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT80130.210420446995738No Hit
GTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAAAGCCCCCAAA80040.2101841080436649No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC78200.20535228946794848No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT77530.20359287726918218No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT73290.19245868663818344No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA71650.18815206573374055No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT71390.18746930876108495No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA71240.1870754105076298No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC70540.18523721865817247No Hit
ACTAGCACCTATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGG70120.18413430354849808No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC69790.18326772739089675No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG69170.18163961460994882No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCT68440.17972264310980046No Hit
CCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAGTAGGTGAATATGAG68180.17903988613714486No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC67920.1783571291644893No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA64490.16934998910214832No Hit
CATTATAACTCCCAACATCAGTGCTGGTTCCAGTGCAGGAGATGGTGATC64200.1685884524788017No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT63210.16598872400599776No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG63070.1656210856361063No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT63010.16546352633472422No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC62740.16475450947850495No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT62260.16349403506744853No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC62220.1633889955331938No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA62210.16336273564963014No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT62190.16331021588250277No Hit
ATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC62190.16331021588250277No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT60730.15947627288220603No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT60340.15845213742322264No Hit
GTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGACTAGAGCTGCAG59750.15690280429296577No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG59680.15671898510802004No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG58390.15333146012830579No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCC58280.15304260140910536No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA58100.15256992350495918No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG57230.15028531363491934No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC57100.14994393514859156No Hit
ATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAG56880.14936621771019068No Hit
GCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACCACCCA55450.14561105436058497No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA55060.1445869189016016No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT54880.14411424099745543No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA54840.14400920146320073No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGG54640.1434840037919272No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC54480.14306384565490837No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT54410.14288002646996265No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC53550.14062167648348647No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCCAGTC53490.14046411718210441No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC53470.14041159741497708No Hit
AACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACC52310.1373654509215906No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.13644635499686195No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA51960.13644635499686195No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC51940.13639383522973458No Hit
ACTCAGGACACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTC51510.1352646602364965No Hit
ACATTATAACTCCCAACATCAGTGCTGGTTCCAGTGCAGGAGATGGTGAT51230.1345293834967136No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC51150.13431930442820417No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG50880.1336102875719849No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC50690.13311134978427505No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTCACCTA50340.1321922538595464No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG49750.13064292072928949No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG49290.12943496608536037No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC49060.12883098876339583No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC49000.12867342946201377No Hit
GTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGC48960.12856838992775907No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC48740.12799067248935816No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA48100.1263100399412829No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAAC48070.12623126029059187No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT47940.12588988180426408No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG47930.12586362192070039No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTCACCTACTAGCA47920.12583736203713672No Hit
ATACCACATAGGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCC47900.12578484227000936No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC47670.1251808649480448No Hit
CTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGACTA47460.12462940739320762No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA47430.12455062774251659No Hit
TTATTACTGCTGCTCATATTCACCTACTAGCACCTATGTGGTATTCGGCG46830.12297503472869602No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC46680.12258113647524087No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC46450.12197715915327632No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGAC46440.12195089926971263No Hit
TCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTT46390.12181959985189425No Hit
GTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGA46040.12090050392716557No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA45420.11927239114621765No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGAACCAGGAGACAACATTATAA44920.11795939696803384No Hit
CATCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAC44880.11785435743377913No Hit
GTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC44670.11730289987894195No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG44570.11704030104330518No Hit
TCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCCAGTCTGCCCTG44490.11683022197479577No Hit
TACTAGCACCTATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAG43940.11538592837879358No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT43470.11415171385130078No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC43320.11375781559784565No Hit
GATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAA42710.11215596270046138No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG42690.11210344293333403No Hit
GGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAG42030.1103702906181314No Hit
CTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT42000.11029151096744037No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT41910.11005517201536727No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTCAGCGGCCCTCAGG41240.10829575981660099No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT41100.1079281214467095No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG40920.10745544354256334No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG40800.10714032493979922No Hit
GCACTGGAACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTC40350.1059586301794338No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG40250.10569603134379703No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC39840.1046193761176863No Hit
CCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGAACCAG39460.10362150054226661No Hit
GCTCATATTCACCTACTAGCACCTATGTGGTATTCGGCGGAGGGACCAAG39070.10259736508328322No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGG38990.10238728601477382No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC38980.10236102613121013No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA38650.10149444997360883No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT38500.10110055172015367No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT38430.10091673253520793No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT38180.10026023544611604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17800.051.8129432
GGTATCA38500.041.9511831
AGCGTCA2700.039.3527223
GTATCAA91150.038.702831
TATCAAC96450.038.160512
ATCAACG103950.035.407233
TCAACGC106600.034.56694
TATACGT1008.072493E-834.000754
CAACGCA109450.033.550315
AACGCAG109900.033.3355876
CAGCGTC3600.031.8757042
TACGTGC1201.12431735E-831.8757026
GACAGGT5000.030.6022831
ACGCAGA123250.029.7228437
CGCAGAG123050.029.7016898
GCAGAGT126750.028.8678079
GCAGCGT3700.028.7183591
ATATCAA7550.028.7107521
GAATACG10300.028.468899
GCTAGAG10650.028.333963
Produced by FastQC (version 0.11.5)