FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808090
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAGTAGGTGAATA177450.4659816338374355No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAG176190.4626728885084123No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC164360.4316074462525833No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCACTGATGTTGGGAGTTATAA152860.4014085801543556No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAC145690.3825802436391997No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCT145120.38108343027607017No Hit
GATTATTACTGCTGCTCATATTCACCTACTAGCACCTATGTGGTATTCGGCGGAGGGACCA141790.37233888904936596No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTG140920.3700542791793261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137930.3622025739937869No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCC131280.3447397514239422No Hit
GTGTTGGAACCAGGAGACAACATTATAACTCCCAACATCAGTGCTGGTTCCAGTGCAGGAG121520.3191101050657941No Hit
GTTCCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTCAGCGGCCC119240.31312285161327597No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC116160.3050348074756637No Hit
CTCCTGCACTGGAACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACAC113680.29852235635187196No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCA111640.293165340104882No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC107370.2819523698231922No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT103850.27270889080877814No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCG97210.2552723281224971No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGC97070.2549046897526056No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCC93450.2453986119025548No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAGTAGGTGAATAT91170.23941135845003664No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA90590.2378882852033434No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAG88930.23352914453177318No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCT87640.2301416195520589No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGG87110.22874984572318408No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC84050.2207143213526991No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCC82540.21674907893458403No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT81810.21483210743443565No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTG81600.21428064987959844No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGAC81300.21349285337268817No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGATCCTGT81080.2129151359342873No Hit
GGACACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCT79530.20884485398191743No Hit
CCCCTGAGGGCCGCTGAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTG74470.19555735289869727No Hit
GTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTA73100.1919597488504736No Hit
CCTATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC70560.1852897384252998No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGC70320.1846595012197716No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCCAGTCT69070.18137701577431206No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG69030.18127197624005734No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA68360.17951256404129104No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGG68060.17872476753438077No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG67610.17754307277401532No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGA67610.17754307277401532No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCAT67200.1764664175479046No Hit
CCACATAGGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTG67170.17638763789721357No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG66460.17452318616419255No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCT63630.16709163911567215No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC62940.1652797071497785No Hit
CCTTGGTCCCTCCGCCGAATACCACATAGGTGCTAGTAGGTGAATATGAGCAGCAGTAATA62110.16310013681399338No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCT61880.1624961594920288No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG61610.16178714263580954No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT60470.15879351590955046No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT58890.1546444543064896No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCCAGTCTGCCCTGACTC58050.15243862408714082No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCA56740.1489985793402992No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGC56560.14852590143615302No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTC55620.14605747238116745No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACC55100.1446919584358563No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGAC54640.1434840037919272No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAAC54590.1433527043741088No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCC54550.1432476648398541No Hit
ATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG54230.14240734856581647No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAA54100.14206597007948865No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG53840.1413832131068331No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGAT53790.1412519136890147No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA53450.14035907764784972No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCG52750.13852088579839236No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTCACCTACTAG52580.13807446777780988No Hit
ACTAGCACCTATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG51840.13613123639409783No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT51560.1353959596543149No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGAC51020.13397792594187638No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCAC51010.1339516660583127No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCAC50940.13376784687336696No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAG50720.13319012943496608No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCAC49850.13090551956492627No Hit
CATTATAACTCCCAACATCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGG49410.1297500846881245No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGG49210.12922488701685098No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGC49050.12880472887983216No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC48950.12854213004419537No Hit
GCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAAAGCCCC48680.12783311318797613No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTCACCTACTAGCACCTATGTGGTA48120.12636255970841023No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCC47030.12350023239996953No Hit
GTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGG46990.12339519286571485No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG46820.12294877484513234No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAA46320.12163578066694852No Hit
GGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCA46200.1213206620641844No Hit
GTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAG45030.11824825568723428No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCC44940.1180119167351612No Hit
ATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTC44910.11793313708447017No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACC44900.11790687720090649No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTCACCTACTAGCACCTAT44720.11743419929676031No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTG44440.11669892255697738No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGC44280.11627876441995856No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGA44060.11570104698155768No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA43600.11449309233762858No Hit
ACTCAGGACACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT43320.11375781559784565No Hit
TTATTACTGCTGCTCATATTCACCTACTAGCACCTATGTGGTATTCGGCGGAGGGACCAAG42670.1120509231662067No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA42130.11063288945376815No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA42070.1104753301523861No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAA41840.10987135283042156No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC41830.10984509294685786No Hit
AACCAGCACTGATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAAA41800.10976631329616685No Hit
GTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCC41680.10945119469340274No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGAACCAGGAGACAACATTATAACTCCCAACATC41570.1091623359742023No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTA41530.10905729643994758No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCC41490.10895225690569288No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGC41480.1089259970221292No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC41360.10861087841936509No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTGGGCCCAGTCTGCCCT41050.10779682202889111No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTT40880.10735040400830863No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATTC40760.10703528540554452No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCAC40650.10674642668634408No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCACCACGGG40450.10622122901507054No Hit
ACATTATAACTCCCAACATCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAG40380.10603740983012482No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTG39680.10419921798066747No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA39570.10391035926146705No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGATCCTG39460.10362150054226661No Hit
ATACCACATAGGTGCTAGTAGGTGAATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC39330.10328012205593881No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCC39070.10259736508328322No Hit
CTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT38890.10212468717913704No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACC38820.10194086799419132No Hit
GATGTTGGGAGTTATAATGTTGTCTCCTGGTTCCAACACCACCCAGGCAAAGCCCCCAAAC38220.10036527498037075No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGT38110.10007641626117028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20700.039.733282
GATCGGA553.4919474E-530.00125350
GCGTCTA400.00784102827.524651
CGGTATG400.00787226527.50223239
GTCCGTT400.00787731327.49862113
GGTATCA44350.026.686811
GTATCAA100050.025.3925551
AGAGTAC61250.025.3211811
CAGAGTA62250.024.91441310
TATCAAC103050.024.2910882
GACAGGT4650.023.6771181
ACGGGTT1052.1021879E-723.57024616
ATAAACG600.002034806222.9197333
GGCGCTA600.002036360822.91672142
GTGCGGT600.002036983122.91551811
ATCAACG108750.022.8122443
TCAACGC110900.022.4396744
CAACGCA112450.022.1294965
AACGCAG113500.022.0438796
ATGATCG752.873792E-422.0006348
Produced by FastQC (version 0.11.5)