FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675020
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99711.4771414180320583No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19280.2856211667802435No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA16990.25169624603715446No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15040.2228082130899825No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA14560.21569731267221715No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA12750.1888832923468934No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA11560.17125418506118337No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCA10890.1613285532280525No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC9680.14340315842493556No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG9380.13895884566383218No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8420.12473704482830138No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGT8340.12355189475867381No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8200.12147788213682556No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT7840.11614470682350152No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA7810.11570027554739118No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCC7670.11362626292554295No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCT7400.10962638144054991No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG7050.10444134988592929No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA6880.10192290598797073No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG6870.10177476222926728No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT6820.10103404343575005No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGT150.00575694655.061791
AGGTACT150.00578233455.00059538
CCTTTAT257.8566186E-443.9972159
TGGTATC19550.037.8505022
GTGGTAT19650.037.8287121
GTCCTAA458.77097E-636.707861
TTACTCC300.001923558436.6724973
CTAACGA300.001924958736.6670646
TCGGAGC300.001924958736.6670653
GAAGTTC300.001924958736.6670655
CAAGGTT300.001925659136.6643455
CCCGCTA458.842619E-636.66434518
CCCACTG558.697152E-734.99778722
CCGCTAT501.8196757E-532.9979119
ACGTGCC501.8196757E-532.997917
AGTAGTA957.094059E-1131.851892
GTGCTAT350.004075981731.463881
AGTCTTC350.0040982331.42891140
CGAGAGT350.0040982331.42891136
CGATAGA350.0040982331.42891150
Produced by FastQC (version 0.11.5)