FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185034
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11820.6388015175589351No Hit
TCTCTGGGCTGCGGACTGAGGATGAGGCTGATTATTACTGCTCCTCATAT10680.577191218911119No Hit
GATTATTACTGCTCCTCATATAAGAGTTACTTCAGTTCCGTCTTCGGCGG9800.5296323918847347No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9510.5139595966146762No Hit
GTAATAATCAGCCTCATCCTCAGTCCGCAGCCCAGAGATGGTCAGGGAGG9430.509636066885005No Hit
GCCTTGGGCTGACCGAGGACGGTCACCCTGGTCCCCCCGCCGAAGACGGA8760.47342650539900777No Hit
GTGTCCGGGTCTCCTGGTCAGTCAATCACCATCTCCTGCACTGGAACCAG8360.45180885675065124No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7810.422084589859161No Hit
GTCAATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGCACTTATT7660.41397797161602734No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7380.3988456175621778No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7030.3799301749948658No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCATCCAG6950.3756066452651945No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGCACTTATTGGTTTGTCTCTT6840.36966179188689646No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6630.3583125263465093No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6250.3377757601305706No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG6210.33561399526573493No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6190.33453311283331716No Hit
CTCTTATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGTCCGCAGCCC6160.3329117891846904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5600.30264708107699123No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA5580.30156619864457346No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA5400.291838256752813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA5030.2718419317530832No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC5030.2718419317530832No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4940.266977960807203No Hit
GTCGAAGACATCATAAATTAAGAGTTTGGGGGCCTTGCCTGGATGTTGTT4820.26049266621269607No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4820.26049266621269607No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG4820.26049266621269607No Hit
GTCTTCGACCGGCCCTCCGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC4680.25292648918577126No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT4640.2507647243209356No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4590.24806251823989103No Hit
ATATAAGAGTTACTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTGACCG4500.24319854729401083No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4190.22644486959153454No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA4160.2248235459429078No Hit
GTGCAGGAGATGGTGATTGACTGACCAGGAGACCCGGACACTGAGCGAGG4150.22428310472669888No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4150.22428310472669888No Hit
CTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTGACCGTCCTCGGTCAGC4090.2210404574294454No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT4070.2199595749970276No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT4040.21833825134840085No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT3990.21563604526735627No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC3950.2134742804025206No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT3890.21023163310526713No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGTCAGTCAATCACCATCTCCTGCA3820.20644854459180473No Hit
GAACTGAAGTAACTCTTATATGAGGAGCAGTAATAATCAGCCTCATCCTC3820.20644854459180473No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA3760.20320589729455127No Hit
GTACCAACAACATCCAGGCAAGGCCCCCAAACTCTTAATTTATGATGTCT3730.20158457364592453No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA3710.2005036912135067No Hit
ATCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC3660.19780148513246215No Hit
GGATGAGGCTGATTATTACTGCTCCTCATATAAGAGTTACTTCAGTTCCG3570.19293751418658192No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGATGGCA3570.19293751418658192No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA3550.1918566317541641No Hit
CTTATTGGTTTGTCTCTTGGTACCAACAACATCCAGGCAAGGCCCCCAAA3440.18591177837586606No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACC3430.18537133715965715No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3420.18483089594344823No Hit
CATATAAGAGTTACTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTGACC3380.18266913107861257No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC3350.18104780742998586No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGTCAGTCAATCACCATCT3330.17996692499756803No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG3270.17672427770031454No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT3240.1751029540516878No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG3220.17402207161926997No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.17402207161926997No Hit
CTTATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGTCCGCAGCCCAG3210.17348163040306105No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC3190.17240074797064325No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC3170.1713198655382254No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC3160.1707794243220165No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGTCAGTCAATCAC3130.16915810067338974No Hit
GAGTTACTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTGACCGTCCTCG3090.1669963358085541No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGATGGCAGATAGCAGCCCCG3080.1664558945923452No Hit
CTCCTGGTCAGTCAATCACCATCTCCTGCACTGGAACCAGCAGTGATATT3040.16429412972750954No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCA3040.16429412972750954No Hit
GGCCTGGATGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA2990.16159192364646496No Hit
GGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC2980.16105148243025608No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT2980.16105148243025608No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT2970.16051104121404716No Hit
GCTATCTGCCATCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA2940.1588897175654204No Hit
GTGATTGACTGACCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGC2930.1583492763492115No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA2930.1583492763492115No Hit
GCCTCATCCTCAGTCCGCAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC2930.1583492763492115No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC2900.15672795270058476No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG2890.15618751148437585No Hit
CCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCCCAGGATCCTG2880.15564707026816693No Hit
GGATGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCC2870.15510662905195802No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC2870.15510662905195802No Hit
ATCAGGGACCCCGGAGGGCCGGTCGAAGACATCATAAATTAAGAGTTTGG2830.15294486418712236No Hit
GACCGAGGACGGTCACCCTGGTCCCCCCGCCGAAGACGGAACTGAAGTAA2820.15240442297091344No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT2820.15240442297091344No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT2820.15240442297091344No Hit
CCGGAGGGCCGGTCGAAGACATCATAAATTAAGAGTTTGGGGGCCTTGCC2810.15186398175470456No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC2810.15186398175470456No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT2780.15024265810607781No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA2760.14916177567365999No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT2750.14862133445745104No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC2740.14808089324124216No Hit
ATATTGGCACTTATTGGTTTGTCTCTTGGTACCAACAACATCCAGGCAAG2700.1459191283764065No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG2680.14483824594398867No Hit
GAAGTAACTCTTATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGTCC2680.14483824594398867No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG2660.14375736351157084No Hit
GCCCTCCGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG2650.14321692229536193No Hit
TCATATAAGAGTTACTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTGAC2650.14321692229536193No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG2640.142676481079153No Hit
GTGCCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGATTGACTGACC2600.14051471621431738No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC2540.1372720689170639No Hit
CTCTTAATTTATGATGTCTTCGACCGGCCCTCCGGGGTCCCTGATCGCTT2530.13673162770085498No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT2530.13673162770085498No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC2520.13619118648464607No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG2510.13565074526843715No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG2500.13511030405222824No Hit
CTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACT2500.13511030405222824No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGG2450.13240809797118366No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC2450.13240809797118366No Hit
CATCCAGGCAAGGCCCCCAAACTCTTAATTTATGATGTCTTCGACCGGCC2420.13078677432255692No Hit
GACTGAGGATGAGGCTGATTATTACTGCTCCTCATATAAGAGTTACTTCA2420.13078677432255692No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC2390.1291654506739302No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC2390.1291654506739302No Hit
AATTAAGAGTTTGGGGGCCTTGCCTGGATGTTGTTGGTACCAAGAGACAA2370.12808456824151238No Hit
CGGTCACCCTGGTCCCCCCGCCGAAGACGGAACTGAAGTAACTCTTATAT2340.12646324459288563No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCCTGGTC2330.12592280337667672No Hit
GGAACCAGCAGTGATATTGGCACTTATTGGTTTGTCTCTTGGTACCAACA2300.12430147972804999No Hit
GACCATCTCTGGGCTGCGGACTGAGGATGAGGCTGATTATTACTGCTCCT2240.12105883243079651No Hit
GTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAC2240.12105883243079651No Hit
TGCCTGGATGTTGTTGGTACCAAGAGACAAACCAATAAGTGCCAATATCA2230.12051839121458759No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA2230.12051839121458759No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11943750878216977No Hit
ATCCAGGCAAGGCCCCCAAACTCTTAATTTATGATGTCTTCGACCGGCCC2210.11943750878216977No Hit
ACCAATAAGTGCCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGATT2200.11889706756596084No Hit
TTACTGCTCCTCATATAAGAGTTACTTCAGTTCCGTCTTCGGCGGGGGGA2200.11889706756596084No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT2200.11889706756596084No Hit
TTGTTGGTACCAAGAGACAAACCAATAAGTGCCAATATCACTGCTGGTTC2190.11835662634975194No Hit
CTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG2190.11835662634975194No Hit
GCCTGGATGTTGTTGGTACCAAGAGACAAACCAATAAGTGCCAATATCAC2180.11781618513354301No Hit
GTCTCTTGGTACCAACAACATCCAGGCAAGGCCCCCAAACTCTTAATTTA2170.11727574391733411No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC2170.11727574391733411No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC2170.11727574391733411No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA2150.11619486148491628No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGTCAGTC2140.11565442026870737No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA2130.11511397905249847No Hit
TCCTCAGTCCGCAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT2120.11457353783628954No Hit
GTTTGGGGGCCTTGCCTGGATGTTGTTGGTACCAAGAGACAAACCAATAA2120.11457353783628954No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACG2110.11403309662008064No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT2070.11187133175524497No Hit
CATCATAAATTAAGAGTTTGGGGGCCTTGCCTGGATGTTGTTGGTACCAA2060.11133089053903607No Hit
GTGATATTGGCACTTATTGGTTTGTCTCTTGGTACCAACAACATCCAGGC2050.11079044932282717No Hit
GAGGATGAGGCTGATTATTACTGCTCCTCATATAAGAGTTACTTCAGTTC2040.11025000810661824No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG2030.10970956689040934No Hit
CCCTGGTCCCCCCGCCGAAGACGGAACTGAAGTAACTCTTATATGAGGAG2030.10970956689040934No Hit
GCACTGGAACCAGCAGTGATATTGGCACTTATTGGTTTGTCTCTTGGTAC1990.10754780202557367No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCATCCAGGCCACTGTCA1980.10700736080936477No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG1970.10646691959315586No Hit
CCTCATATAAGAGTTACTTCAGTTCCGTCTTCGGCGGGGGGACCAGGGTG1970.10646691959315586No Hit
GTCCGGGTCTCCTGGTCAGTCAATCACCATCTCCTGCACTGGAACCAGCA1950.10538603716073802No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC1950.10538603716073802No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC1940.10484559594452912No Hit
CTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCA1910.10322427229590236No Hit
ACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGA1910.10322427229590236No Hit
GAGCAGTAATAATCAGCCTCATCCTCAGTCCGCAGCCCAGAGATGGTCAG1910.10322427229590236No Hit
GGGCTGCTATCTGCCATCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC1880.10160294864727563No Hit
CCGTGACAGTGGCCTGGATGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG1870.10106250743106672No Hit
GTACCAAGAGACAAACCAATAAGTGCCAATATCACTGCTGGTTCCAGTGC1870.10106250743106672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTA203.0180709E-585.021366
GGTTACT150.001028263484.998388
TCAATAG200.003216002863.766022
AGGTTAC200.003219450663.7487877
TGGTATC1000.055.263882
GTGGTAT1050.052.632271
ACAGGGT250.00777824751.0128142
AATAGGT250.00777824751.0128144
GTATCAA4900.041.6431161
AGACAGG450.001657394137.787272
TATCAAC5600.037.1968462
ATCAACG5800.035.9141923
TCAACGC6000.034.7170524
GGTATCA2100.034.4134061
CAACGCA6150.033.8702965
GAATATT1157.3268893E-933.2692261
AACGCAG6300.033.063866
GACAGGG550.00443300830.9168571
CGCAGAG6900.030.1805828
ACGCAGA7100.029.3304277
Produced by FastQC (version 0.11.5)