FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707853
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44130.6234345266601964No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC9590.13548010674532707No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9160.1294053991436075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15100.035.8622782
GTGGTAT15350.035.477331
GTCTTAT1203.6379788E-1127.503891
ACCACGG400.00786840727.50194520
AGTGTCG400.00787655327.4961138
GACCGTG507.043763E-427.4902847
TTCCGTA507.043763E-427.4902847
ACAGCGT400.007884704527.4902846
GTATCAA58000.025.9864331
AGACGCA1001.3136014E-724.739512
AGAGTAC39850.023.67001311
GGTATCA29900.023.6404671
CGAACTA701.7996703E-423.57142829
CAGAGTA40550.023.26140610
TATCAAC65550.023.1061442
TATACTG600.002038945222.9085715
GCGAACT752.8711467E-422.028
AGCGAAC903.9928345E-521.3888927
GTATTGG650.003235880321.1568371
CTAGACA1459.713403E-920.85474
Produced by FastQC (version 0.11.5)