FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245829
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC139050.6191477623630294No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT122790.5467468805505673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA114460.5096558998926455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC109640.4881938918768971No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC100680.44829771100114923No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG86760.3863161442834695No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG81950.36489866325530573No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTAC81640.363518326640185No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC80420.3580860341548711No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG78790.35082813517859107No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC78360.3489134747124559No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT75190.33479841964815665No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT73940.3292325462000892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG73240.32611565706917134No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC72270.32179653927347096No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA68510.3050543919416839No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC68000.3027835155748723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT67810.3019375028107661No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT65420.291295552778061No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGA64880.2888910954484959No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCG62950.2802973868446796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT59640.2655589539541969No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG58460.26030476941922115No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG57870.2576776771517333No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA56340.25086504805129867No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC55780.2483715367465644No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC52760.23492438649603328No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT52630.2343455356574343No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT52160.2322527672409609No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTG50380.2243269634509128No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC50020.22272399189786932No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA49970.2225013569599466No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC49410.22000784565521242No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCAGCTGGATGT48000.21372954040579226No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA47850.21306163559202412No Hit
GGGTGGAAGGAGACTGGGTCAGCTGGATGTCACATTTGGCACCTGGGAGC47210.21021190838661358No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT46550.20727312720603394No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTA46550.20727312720603394No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC46160.2055365746902369No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT45780.20384454916202435No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA45630.20317664434825627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT45150.20103934894419836No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA44900.1999261742545848No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC44480.19805604077603417No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA43830.19516178658303907No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC43720.1946719897196091No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA43270.1926682752783048No Hit
ACCTTGATGGGACCCCACTTTCCAAACTAGATGCCTTATAGATCAGGAGT43170.1922230054024594No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC42900.1910207767376768No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC42140.18763672568125178No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCA41840.18630091605371557No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC41350.18411909366207313No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAAC40760.18149200139458527No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC40160.17882038213951285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG40070.17841963925125198No Hit
TTCCAAACTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT39180.17445673735622794No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCAGCTGGATGTCACATTTGGC39140.17427862940588976No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38920.17329903567902988No Hit
GAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCA38730.17245302291492362No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA38370.17085005136188017No Hit
GTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTT38330.17067194341154202No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT38300.1705383624487884No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC38260.17036025449845024No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT38170.16995951161018938No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG37750.16808937813163868No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA37310.16613019067791893No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC37180.1655513398393199No Hit
GTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA36780.1637702603359383No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA36550.1627461396214939No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC35600.1585160758009626No Hit
TTAGTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCT35290.15713573918584184No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34640.15424148499284673No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC33820.15059027201091446No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC33810.15054574502332993No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT33800.15050121803574537No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC33490.14912088142062463No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT33110.14742885589241211No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT33010.1469835860165667No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT32820.14613757325246046No Hit
AGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCCGGGAC31660.1409724426926538No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA31320.13945852511477944No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC31310.1394139981271949No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA31200.13892420126376495No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT31010.1380781884996587No Hit
GCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCGGCAAGTG30920.13767744561139786No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG30470.13567373117009354No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG30460.13562920418250898No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG30320.13500582635632544No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC30260.1347386644308182No Hit
CAGTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAAT30120.13411528660463465No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCA29950.13335832781569745No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC29890.1330911658901902No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG29590.13175535626265403No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC29110.1296180608585961No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT29070.12943995290825794No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28790.1281931972558908No Hit
CATCAAGGTTCAGCGGCAGTGGATCCGGGACAGAATTCACTCTCACCATC28440.12663475269043192No Hit
CTCCTGATCTATAAGGCATCTAGTTTGGAAAGTGGGGTCCCATCAAGGTT28340.1261894828145865No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT28050.12489820017463485No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA27850.12400766042294405No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC27730.12347333657192956No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG27690.12329522862159141No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT27300.12155867610579435No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT27220.12120246020511803No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC27090.120623609366519No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAGTCTCCTTCCACC26990.12017833949067361No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG26930.11991117756516637No Hit
CTATAAGGCATCTAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCA26850.11955496166449005No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG26750.11910969178864463No Hit
CCCCTAAACTCCTGATCTATAAGGCATCTAGTTTGGAAAGTGGGGTCCCA26650.11866442191279925No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC26100.11621543759564953No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA26060.11603732964531137No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC25800.11487962796811334No Hit
CCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAG25530.11367739930333075No Hit
GGTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTA25380.11300949448956264No Hit
ACCAGGTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCT24940.11105030703584289No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24840.11060503715999749No Hit
ACAGTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAA24540.10926922753246128No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG24290.1081560528428478No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT24240.10793341790492508No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC24220.107844363929756No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT24210.10779983694217148No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA24110.10735456706632605No Hit
GGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCG24100.10731004007874152No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAGTCTCCTT24000.10686477020289613No Hit
GAAGGAGACTGGGTCAGCTGGATGTCACATTTGGCACCTGGGAGCCAGAG23830.10610781141395895No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTG23630.10521727166226814No Hit
GTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAA23580.10499463672434543No Hit
CTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTG23540.10481652877400728No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAGTC23130.10299092228304113No Hit
CTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGT23080.10276828734511845No Hit
GGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCC23010.10245659843202666No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGG22630.10076457290381415No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC22550.10040835700313783No Hit
TACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATGCA22510.10023024905279965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8100.060.8787231
GTACGAC250.00779123551.00993
GCAGCGT2350.041.605671
GTATCAA46000.039.090851
GACAGGT4500.037.7867971
ATACCGA450.001661799337.785116
TATCAAC49200.037.4971162
ATCAACG53200.034.7576873
TCAACGC54350.034.1004564
CAACGCA55100.033.5591435
AACGCAG56800.032.6295746
TATACGT550.0044446830.915094
CGCAGAG60600.030.5065568
GCCTGAC18750.030.1458077
ACGCAGA61700.030.0315577
GCAGAGT63500.029.0457679
GTCGAGG6300.029.0071098
AAGTACG755.899179E-428.3400971
GGTACGG600.006800495628.3388373
TCTAAGG755.900716E-428.3388353
Produced by FastQC (version 0.11.5)