FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245829
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTG101270.45092480326863715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGA98410.4381900848194587No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC95410.42483198854409665No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACA95060.4232735439786377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTG94130.41913253413327545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG73880.3289653842745819No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCT73440.3270061968208621No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCA70720.3148948561978672No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATGCAGACA70060.3119560750172876No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAG68950.30701357939540364No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC66570.2964161563502831No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCAC65510.2916962956663219No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCC63680.28354785693835105No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCA63650.28341427597559743No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTG61470.2737073926821677No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGAT61250.2727277989553078No Hit
GGGTGGAAGGAGACTGGGTCAGCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAG60630.2699671257250663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC60040.2673400334575785No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG59290.264000509388738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA54780.24391883798811043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG54470.24253850137298966No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCA53340.23750695177593661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53180.23679451997458398No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATGCAGACAGGG52960.23581492624772413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC52410.23336594193057442No Hit
GAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT52240.23260898314163722No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA50470.22472770633917366No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGC48600.21640115966086465No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTACC48360.21533251195883568No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCTGAAATAATT47860.21310616257960868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGG47480.21141413705139614No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA43860.19529536754579266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC43470.1935588150299956No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC42990.19142151962593768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACT42870.19088719577492322No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAAC42390.18874990037086528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTG42340.18852726543294257No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCAGCTGGATGTCACATTTGGCA42240.1880819955570972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG41440.18451983655033397No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT41220.1835402428234741No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC40620.1808686235684017No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCT40590.18073504260564807No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGG40450.18011166477946453No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTG40440.18006713779187997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGT39910.17770720744989935No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGG38700.17231944195217No Hit
GTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG38520.17151795617564827No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACT37840.16849012101989955No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT37240.16581850176482715No Hit
GTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAG36920.16439363816212188No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT36710.16345857142284653No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCC36680.1633249904600929No Hit
TTCCAAACTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATAC36460.16234539673323303No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAG36440.16225634275806394No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTG36210.16123222204361953No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAAC35900.1598518854284988No Hit
ACCTTGATGGGACCCCACTTTCCAAACTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTT34280.1526385134398033No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAG34220.15237135151429604No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAC34030.1515253387501898No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCAGCTGGATGTCACATTTGGCACCTGGGAGCC33200.14782959878067298No Hit
TTAGTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGA33120.14747338287999665No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATA33110.14742885589241211No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG33000.14693905902898216No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG32010.1425308872581127No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGT31950.14226372533260545No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATC31850.14181845545676006No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAGTATACTAAT31770.14146223955608375No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACT31270.13923589017685672No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31040.13821176946241231No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTC30960.137855553561736No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT30810.1371876487479679No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG30600.13625258200869256No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTC30390.13531751526941724No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACA30270.13478319141840273No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGA29570.13166630228748494No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC29490.13131008638680863No Hit
GCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCT28160.12538799703806477No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTT28130.12525441607531118No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC28120.12520988908772662No Hit
CAGTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG28030.12480914619946576No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAG27980.12458651126154308No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACC27470.12231563489473153No Hit
GGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTG27080.12057908237893447No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCT27070.12053455539134993No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGA26410.11759577421077028No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC26030.11590374868255776No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCT26000.11577016771980413No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25860.11514678989362058No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTG24960.11113936101101198No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA24840.11060503715999749No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA24730.11011524029656755No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGG24710.11002618632139846No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAA24670.10984807837106031No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAAT24670.10984807837106031No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT24030.10699835116564974No Hit
AGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCCGGGACAGAATTCACTC24000.10686477020289613No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGT23960.10668666225255796No Hit
CTGTTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCC23860.10624139237671257No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTC23800.10597423045120531No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGA23480.10454936684850004No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCATCTAGTTTGGA23430.10432673191057733No Hit
GGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCGGCAAGTGATGC23350.10397051600990101No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTAC23250.10352524613405562No Hit
GTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACTAGATGCCTTAT22970.1022784904816885No Hit
ACAGTATACTAATTATTTCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT22850.10174416663067402No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCC22750.10129889675482862No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAG22740.10125436976724407No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCT22680.10098720784173683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10000.044.5837251
GACAGGT3600.028.2852861
TGCCGAC3100.027.50181655
GTATCAA52050.026.5426541
GTAATAT2200.025.0189251
AGCCGTG2100.024.88093410
TATCAAC55300.024.5687182
TATACGC701.8005623E-423.57298355
ATCAACG59150.022.8725013
AGAGTAC38300.022.8328811
CAACGCA59900.022.6743975
CGACGAC852.570422E-522.64855255
AATATAC2550.022.6455273
TCAACGC59700.022.6137084
AACGCAG61150.022.255866
CCGTGTC2400.021.77081512
AAGCGCT1155.0474773E-721.5217249
CGGGAGT11150.021.45594817
ACCGCTA650.003239460321.15524355
GTGCGCG650.003241971421.15241616
Produced by FastQC (version 0.11.5)