FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468549
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24820.5297204774740741No Hit
GTATTACTGTCAGCGGTATGGTAGCTCGGTCACTTTCGGCGGTGGGACCAAGGTGGAGATC18610.39718364568060116No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT18370.39206144928278575No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCAGTCG15820.33763811255599735No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG14980.3197104251636435No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTG13370.2853490243282986No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG13350.2849221746284807No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA11780.2514144731927717No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA10360.22110814450569735No Hit
GTGTATTACTGTCAGCGGTATGGTAGCTCGGTCACTTTCGGCGGTGGGACCAAGGTGGAGA10020.21385169960879227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACA9590.20467443106270636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9520.2031804571133435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG9200.19635086191625636No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCAGTCGCA9100.19421661341716662No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTG8800.18781386791989738No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTG8400.17927687392353842No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCT8340.17799632482408456No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC8340.17799632482408456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCAC8090.1726607035763602No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGA7810.16668480777890893No Hit
GAGTATTAGCAGCAGCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTC7760.16561768352936407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG7740.1651908338295461No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCC7680.16391028473009228No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGA7510.16028206228163971No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC7490.15985521258182175No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGAC7390.157720964082732No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTG7360.15708068953300508No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAG7170.15302561738473458No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTC7020.14982424463609997No Hit
GTATGGTAGCTCGGTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCT6960.14854369553664612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6880.1468362967373743No Hit
CATTAGGATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACATTTGTT6880.1468362967373743No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTA6700.1429946494390128No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCT6610.141073825789832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC6510.13893957729074227No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGCTGC6470.13808587789110638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCA6350.1355247796921987No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTATTTAGCCTGGTACCAGCAGAAACCT6110.13040258329438328No Hit
GAGATATTCTCCCTGTTGTTCAGAGGAACAATAATTCGGATGTTTCTCTCCACAATGTCCT6000.12805490994538457No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATC5960.12720121054574868No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAA5950.1269877856958397No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTGACCGAGCTACC5880.1254938117464769No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCG5800.12378641294720509No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCC5730.12229243899784228No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCAGAGATATTCTC5640.1203716153486615No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCT5580.11909106624920768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCT5560.11866421654938972No Hit
TTATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA5530.11802394199966278No Hit
GATGAAGACAGTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTG5480.1169568177501179No Hit
CTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGTACACAAATCTGGTTCTCAA5390.11503599410093715No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTG5340.11396886985139228No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCA5330.1137554450014833No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCT5300.11311517045175638No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAA5280.11268832075193842No Hit
ATGGTAGCTCGGTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGC5270.11247489590202947No Hit
CTACAGAAGTGGAGCTGGATAATCAGATAGTTACTGCTACCCAGAGCAATATCTGTGATGA5160.11012722255303074No Hit
GAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGACAGAAACAAG5150.10991379770312176No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGA5120.10927352315339484No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCC5120.10927352315339484No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA5110.10906009830348587No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAATCAGATAGTTAC5070.10820639890384996No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCA5020.10713927465430509No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.10671242495448716No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGAGAATA4900.10457817645539741No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACT4900.10457817645539741No Hit
GTATCAACGCAGAGTACGGGAGAAGAAGTGAAGTCAAGATGAAGAACCATTTGCTTTTCTG4900.10457817645539741No Hit
GTATTAGCAGCAGCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT4880.10415132675557946No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGT4880.10415132675557946No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGA4830.10308420250603459No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGC4820.10287077765612561No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGG4780.10201707825648972No Hit
CACTTACATTTGTTGTCAACAAGAACAATCCTTTCATCTTCTTGGGCTTTCACATGAACAG4710.10052310430712688No Hit
CCATTTGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAATCAGATA4690.10009625460730895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGC150.00576116155.0489541
GATTCAC305.388574E-755.0489541
ACTTGAG150.00578067955.00192342
GTACGCG150.00578067955.00192314
CGCGCGT150.005783122554.99604830
GCGTTAG150.005783122554.99604833
CGTTAGA150.005783122554.99604834
TGCGAAC150.005783122554.99604829
CGCGTTA150.005783122554.99604832
TGCTTAA150.005785566354.99017719
GACAGGC553.8016879E-1045.040051
ATTCACT403.939698E-641.2426342
CGGGGCT551.9272193E-839.9928617
GATGTGT300.001924129136.6679513
AGTTCGG300.001924129136.6679510
TTCAGCG300.001926147836.6601217
ACAGGCC300.001926147836.660122
AAGATTA350.004094334331.43302537
CCGCTAC350.004096475431.42966810
CGCTACA350.004096475431.42966811
Produced by FastQC (version 0.11.5)