FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217018
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67253.09882129592937No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11150.5137822669087357No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.35665244357610887No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.2889161267728944No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.20827765438811524No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.16588485747725995No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA3580.1649632749357196No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.1391589637725903No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA2930.13501184233565877No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC2640.12164889548332396No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.1211881042125538No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC2620.12072731294178363No Hit
GTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.11796256531716262No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.11750177404639248No Hit
GGTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.11335465260946098No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAA2390.1101291137140698No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.10966832244329963No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCT2240.1032172446525173No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGAC150.00575071455.064161
AAGAGAT150.00577702355.0006954
CGCTGCT150.00577702355.000697
TACTGCG150.00577702355.0006937
GAGGGGC150.00577702355.0006955
AGTCCTA557.2759576E-1250.0006329
CCAATGC501.4915713E-1049.5006218
CAATGCG501.4915713E-1049.5006219
GCGACGG303.4815308E-545.83390844
GAGTCCT601.8189894E-1145.83390828
ATCGTCA601.8189894E-1145.83390810
TACTAGG601.8189894E-1145.8339082
ACGAGTC601.8189894E-1145.83390826
GTACGTG257.841862E-444.00055314
GTCCTAC654.1836756E-1142.30822430
TCCTACA654.1836756E-1142.30822431
CACTATC654.1836756E-1142.30822436
CTACACT654.1836756E-1142.30822433
ACACTAT654.1836756E-1142.30822435
ACTAGGC654.1836756E-1142.2887273
Produced by FastQC (version 0.11.5)