FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109049
Sequences flagged as poor quality0
Sequence length91
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT16631.5250025218021257No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11501.0545717979990648No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.7427853533732542No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.5988133774725124No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.36130546818402737No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA3600.33012682372144636No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.32829278581188276No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.3145375014901558No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.2943630844849563No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.29069500866582915No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.27969078120844754No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.23200579555979423No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA2410.2210015681024127No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.21183137855459475No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.1944080184137406No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.1916569615493952No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.18798888573026803No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.17148254454419573No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC1820.16689744977028675No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1770.16231235499637778RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1650.15130812753899622No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.14764005171986905No Hit
GGTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAA1600.14672303276508725No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.14122091903639647No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT1530.1403039000816147No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.1403039000816147No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1520.1393868811268329No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.1375528432172693No Hit
TATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAA1460.13388476739814212No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.13021669157901494No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.12563159680510597No Hit
ATATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA1340.12288053994076059No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.11554438830250621No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1260.11554438830250621No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1230.11279333143816082No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1200.11004227457381544No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1150.10545717979990646No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA1140.10454016084512467No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.10454016084512467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)