FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485685
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCA162510.6537835646914231No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA144540.5814896095040201No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTG132720.5339373251236581No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT122900.49443111255046396No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA114950.4624479771169717No Hit
GTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCGGCAGCAG110740.44551099596288346No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT106030.4265624968570032No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC105720.4253153557268922No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA104770.42149347161848744No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG96550.3884241164910276No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC95460.3840390073561211No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC94550.3803780446838598No Hit
GAGTGGGGCTGAGTCAGCATTAAATTGGCCCAAGAACCTGTGCAGTGAGC94430.3798952803754297No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG91900.3697169995393624No Hit
GTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGAGGGACCAAGCTGA90500.36408474927434487No Hit
GACTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGG88060.35426854166960015No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC81780.3290038761950931No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA78470.31568762735423034No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA75620.3042219750290161No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGC74830.3010437766651849No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA73650.29629659429895583No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC73060.2939230031158413No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGC71220.2865206170532469No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT69500.27960099529908256No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA69490.27956076494004667No Hit
CTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGAG68080.2738882843159934No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGCCAGCAACTATGTGCAGTGGT67630.2720779181593806No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA67370.27103192882444876No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT67230.2704687037979471No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG67060.2697847876943378No Hit
ATTTAATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACG64620.259968580089593No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT63590.2558248531089016No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA63570.25574439239082986No Hit
CTGCACAGGTTCTTGGGCCAATTTAATGCTGACTCAGCCCCACTCTGTGT63530.25558347095468653No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT61900.24902592243184474No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG60740.2443592007836874No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA57730.2322498627138998No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG57470.23120387337896797No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGA56090.22565208383202215No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC55540.223439414085051No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATTAAATTGGCCCAAGAACCTG55380.2227957283404776No Hit
GTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTC54160.2178876245381052No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT53500.2152324208417398No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT51980.20911740626829226No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT51250.206180590058676No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT51090.20553690431410254No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGAT50540.2033242345671314No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC50250.20215755415509207No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA49560.19938165938161914No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA48080.19342756624431495No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA46640.1876343945431541No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGTCCGGGCGC45980.1849791908467887No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA45430.18276652109981756No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC44950.18083546386609728No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG44060.1772549619119076No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT43520.17508252252397227No Hit
GTGCAGGAGATGGTTACCGTCCTCCCCGGAGACTCCGACACAGAGTGGGG43510.1750422921649364No Hit
GTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGG42690.1717434027239976No Hit
GGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGAT42250.16997326692642067No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA42110.16941004189991893No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTAATGCT41940.16872612579630966No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAAT41610.16739852394812696No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT41440.1667146078445177No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT41420.16663414712644603No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG41270.16603069174090843No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCA41230.1658697703047651No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT40590.1632950273264714No Hit
GCACAGGTTCTTGGGCCAATTTAATGCTGACTCAGCCCCACTCTGTGTCG40390.1624904201457546No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA39860.1603582111168551No Hit
CTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCT39610.15935245214095914No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT39490.15886968783252906No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTG39330.15822600208795565No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC39050.15709955203495216No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG39010.15693863059880878No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC38780.15601333234098447No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGA38280.15400181438919253No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA37850.15227190895065143No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA37760.15190983571932887No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA37620.15134661069282712No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA37240.14981785704946524No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT36940.14861094627839005No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTG36930.14857071591935422No Hit
GGTTCTTGGGCCAATTTAATGCTGACTCAGCCCCACTCTGTGTCGGAGTC36260.145875281863953No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG36140.14539251755552293No Hit
GATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTG35960.1446683710928778No Hit
GCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGCTATCATAAGACTGA35780.1439442246302327No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA35720.1437028424760177No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT35180.14153040308808235No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC35170.14149017272904651No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC34810.14004187980375632No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT34690.13955911549532624No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC34660.13943842441821874No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG34370.13827174400617936No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC34360.13823151364714353No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC34210.13762805826160596No Hit
CTCCGGGGAGGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATT34020.13686368143992503No Hit
CGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACC33990.1367429903628175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC33960.13662229928570999No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC33670.13545561887367064No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC33030.13288087589537692No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC33010.13280041517730526No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32960.13259926338212605No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG32820.1320360383556243No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC32190.12950152573636642No Hit
CCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCT32180.1294612953773306No Hit
CTTCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC31780.12785208101589704No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT31660.12736931670746696No Hit
CTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACT31530.12684632204000104No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG30880.12423134870267151No Hit
CCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGA30370.1221796003918437No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAG29970.12057038603041012No Hit
CCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTG29760.11972554849065752No Hit
CCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCTCCTCCAA29540.11884048059186904No Hit
GCATTAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGT29160.11731172694850717No Hit
CTCACTGCACAGGTTCTTGGGCCAATTTAATGCTGACTCAGCCCCACTCT29030.11678873228104124No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG28980.11658758048586206No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC28970.11654735012682621No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC28790.1158232036641811No Hit
GGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGG28770.11574274294610942No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG28750.11566228222803775No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA28610.115099057201536No Hit
CTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCA28370.11413352858467586No Hit
GTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAGGGTCTTTGGTTAT28300.11385191607142497No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28180.11336915176299492No Hit
GCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGA28010.11268523565938565No Hit
ACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCA27980.11256454458227813No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC27870.1121220106328839No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG27480.11055302663048616No Hit
CTATGTGCAGTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGA27460.11047256591241447No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG27460.11047256591241447No Hit
AGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAG27330.10994957124494857No Hit
GTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGATA27260.10966795873169771No Hit
AGGCTGACTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTC27090.10898404262808845No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG27070.10890358191001676No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGT26850.10801851401122829No Hit
CCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCTCCTCCAAC26690.10737482826665486No Hit
GCCAGCAACTATGTGCAGTGGTACCAGCAGCGCCCGGACAGTGCCCCCAC26320.10588630498232883No Hit
GTGCAGTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTA26300.10580584426425714No Hit
GTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGCCAGCAACTATGT26120.10508169780161203No Hit
ACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATT25830.1039150173895727No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT25790.10375409595342934No Hit
ACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTAATGC25770.10367363523535765No Hit
CCTCGCTCACTGCACAGGTTCTTGGGCCAATTTAATGCTGACTCAGCCCC25750.10359317451728597No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT25540.10274833697753337No Hit
CAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT25530.10270810661849752No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG25490.10254718518235416No Hit
CCCAATTGAAGTTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC25290.10174257800163737No Hit
CACCATCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGT25260.10162188692452985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)