FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485685
Sequences flagged as poor quality0
Sequence length61
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179270.7212096464354896No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGC132800.5342591679959448No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGCTATCATAAGA118460.47656883313855136No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC110480.44446500662795163No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATC110330.44386155124241405No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACTT108350.4358959401533179No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAC98710.3971138740427689No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTG90860.3655330421996351No Hit
GTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT87770.35310186125756077No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCC87560.3522570237178082No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAG86980.3499236628937295No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCGGCAGCAGT86200.34678569488893407No Hit
GAGTGGGGCTGAGTCAGCATTAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGA83190.3346763568191464No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCT79400.31942905074456335No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT79090.31818190961445236No Hit
GACTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGAGGGACCAAGC77750.3127910415036499No Hit
GTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTG75420.30341736784829937No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC74930.30144608025554326No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC74550.29991732661218135No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCG64600.25988811937152134No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCTCCTCCAACTC64520.25956627649923464No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGC63770.2565489995715467No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGCTATCATAAGAC62470.2513190528968876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA61480.24733624735233947No Hit
CTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGAGGGACCAAGCTG59680.24009478272588844No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAG59680.24009478272588844No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGAC57350.2307211090705379No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC57180.23003719296692862No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCC56810.2285486696826026No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAGGGTCTTTGGT56500.22730152855249156No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGTCCGGGCGCTGCTGGTACCA55560.22351987480312266No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCT55400.22287618905854925No Hit
GTCCGGGCGCTGCTGGTACCACTGCACATAGTTGCTGGCAATGCTGCCACTGCTGCCGGTG55360.2227152676224059No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGG55270.22235319439108336No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55110.2217095086465099No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGG54560.21949683889953875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTG52810.21245652606826693No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGG50880.20469206677434992No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGA49830.2004678790755868No Hit
GTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATT48850.19652530389007455No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATTAAATTGGCCCAAGAACCTGTGCAGTGAGCG46770.18815738921062003No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCAT46750.18807692849254834No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGCCAGCAACTATGTGCAGTGGTACCAGCAGCGC46290.18622633197689975No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGC46150.18566310695039798No Hit
CTGCACAGGTTCTTGGGCCAATTTAATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCG45870.18453665689739449No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG44020.17709404047576421No Hit
GTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGA44010.17705381011672838No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG43430.1747204492926497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG42820.17226639739146352No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCA42370.17045603123485076No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCT42280.1700939580035282No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGC41040.16510539348308415No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCT41020.16502493276501246No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAG40780.16405940414815232No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC40640.16349617912165057No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGTCCGGGCGCTG40070.16120304865660773No Hit
GTGCAGGAGATGGTTACCGTCCTCCCCGGAGACTCCGACACAGAGTGGGGCTGAGTCAGCA39940.1606800539891418No Hit
GTGGGGGCACTGTCCGGGCGCTGCTGGTACCACTGCACATAGTTGCTGGCAATGCTGCCAC39090.15726047347109548No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCC38750.15589264126387697No Hit
ATTTAATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAGGACGGTAACCATCTC38360.15432365726147923No Hit
GCTGGTACCACTGCACATAGTTGCTGGCAATGCTGCCACTGCTGCCGGTGCAGGAGATGGT37160.14949601417717853No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACC36890.14840979448321084No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGG36420.14651896760852642No Hit
GTGGTGGGGGCACTGTCCGGGCGCTGCTGGTACCACTGCACATAGTTGCTGGCAATGCTGC36150.14543274791455876No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA35690.14358215139891017No Hit
GGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACC35630.1433407692446951No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAA35420.1424959317049425No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGAC35280.14193270667844077No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGC35140.141369481651939No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCAC34920.14048441375315054No Hit
GCTTGGTCCCTCCGCCGAACACCCAATTGAAGTTGCTATCATAAGACTGACAGTAGTAGTC34690.13955911549532624No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT34480.13871427795557362No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAA34170.1374671368254626No Hit
CTGCTGGTACCACTGCACATAGTTGCTGGCAATGCTGCCACTGCTGCCGGTGCAGGAGATG34110.13722575467124756No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT34080.13710506359414004No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTA34070.1370648332351042No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC34060.13702460287606835No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCAC33660.1354153885146348No Hit
CTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGC33490.13473147241102554No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGG33340.13412801702548793No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC32880.13227742050983934No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA31850.12813369352914789No Hit
GATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGAC31180.12543825947374668No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACC30970.12459342193399404No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAA30720.12358766295809807No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGG30610.12314512900870384No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTT30280.12181752716052113No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA30190.1214554539291986No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG30180.12141522357016275No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG30100.12109338069787605No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAA30040.12085199854366101No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTG30020.12077153782558932No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGA29990.1206508467484818No Hit
TCCTCATAGATCACAGTGGTGGGGGCACTGTCCGGGCGCTGCTGGTACCACTGCACATAGT29960.12053015567137428No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGA29770.11976577884969335No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCC29210.11751287874368635No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA29200.1174726483846505No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG29130.11719103587139965No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGC28980.11658758048586206No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCA28810.11590366438225279No Hit
GCACATAGTTGCTGGCAATGCTGCCACTGCTGCCGGTGCAGGAGATGGTTACCGTCCTCCC28500.11465652325214178No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGC28440.11441514109792672No Hit
GTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAGGGTCTTTGGTTATCCTCATAGATC28430.11437491073889089No Hit
GGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACTTCAATTGGGTGTTCGGCGGA28330.1139726071485325No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTAATGCTGACTC28040.11280592673649317No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGG27850.11204154991481222No Hit
CGCCCGGACAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCC27820.1119208588377047No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTA27740.11159901596541799No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCAC27740.11159901596541799No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCC26780.10773690149797743No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACC26610.10705298539436815No Hit
CCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTC26380.10612768713654384No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA26200.10540354067389873No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTAATGCTGAC26180.10532307995582706No Hit
CTTCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC26150.10520238887871955No Hit
CCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCTCCTCCAACTCT26070.10488054600643283No Hit
GTTGGAGGAGCTGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAGGGTCTTTGGTTA25990.10455870313414613No Hit
GCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGA25920.10427709062089524No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAG25450.1023862637462108No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTA25420.10226557266910327No Hit
CCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCC25250.101581656565494No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCG25030.10069658866670556No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)