FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862127
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC234070.8178183567675369No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT217470.7598195328159791No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC192090.6711442224611277No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA184410.6443110316208889No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC176550.6168489378703321No Hit
GAGTTACACCCACTCGCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAA167070.5837267179269124No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC140910.49232616162734916No Hit
GATCTATGCTGCATCCAATTTGCGAGGTGGGGTCCCATCAAGATTCAGTG139850.48862262226658704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG138590.4842203018943603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG136300.4762192593130913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG134840.4711181579294001No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT129390.4520763753669911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG123470.4313924574276404No Hit
GCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATT123240.4305888592644561No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT112100.39166675692588065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT111250.3886969376271563No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG109390.382198274220536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT108030.37744656334257704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA104240.3642046631753238No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC102850.3593481351456452No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC102790.35913850084220583No Hit
CACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC98200.3431014766290944No Hit
ATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTGCTAC94480.33010414981585373No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC94090.3287415268434979No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG91200.31864414122783513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA89670.31329846649013127No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG87710.3064504125777787No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT86770.30316614182389534No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATT86510.3022577265089914No Hit
ACTTACTACTGTCAACAGAGTTACACCCACTCGCCGTGGACGTTCGGCCA84080.2937675372196971No Hit
GATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAAT83020.290063997858935No Hit
GTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACTT82160.2870592395096374No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC80960.2828665534408501No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT80650.28178344287308005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT79840.27895337977664864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC79800.27881362357435574No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC79070.2762630728825101No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA79050.27619319478136367No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT77500.27077764194251336No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC75960.2653970281542363No Hit
GTCTCCAATAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT74690.26095976873143645No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC74510.26033086582111836No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA72610.2536924462122051No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA72270.2525045184927154No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT70920.24778774666532966No Hit
CTCCTGATCTATGCTGCATCCAATTTGCGAGGTGGGGTCCCATCAAGATT70690.24698414850214545No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCAATTTGCGAGGTGGGGTCCCA70310.24565646458036278No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG69740.2436649386976888No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA69490.2427914624333581No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC68310.2386686544657173No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGGTGATGGTGAGAGTGA66870.23363743118317248No Hit
GCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATAAGTAGCA65710.22958450131667812No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA64560.22556651050075696No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG63880.2231906550617775No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC63620.22228223974687356No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC62760.21927748139757597No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA61380.21445589241847063No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC60730.2121848541312108No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA60520.21145113406917304No Hit
GCTCTCACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCAATAGATGCAG59370.20743314325325185No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT58640.20488259256140626No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC57750.201773017060389No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC57240.19999112548115439No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT56910.1988381368122379No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG56840.1985935634582253No Hit
GCAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAATT56720.19817429485134655No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT56520.19747551383988202No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC55550.19408642593427894No Hit
CATCAAGATTCAGTGGCAGCGGATCTGCGACTGATTTCACTCTCACCATC55420.19363221827682697No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA53360.18643477385874213No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC53010.18521190708867916No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC52120.1821023315876619No Hit
GATTTCACTCTCACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTA51600.1802855009578541No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT51500.1799361104521218No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC50720.17721086450741005No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG50370.17598799773734708No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG50000.17469525286613766No Hit
ATCTTGATGGGACCCCACCTCGCAAATTGGATGCAGCATAGATCAGGAGC49200.17190012882027947No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA49000.17120134780881494No Hit
ATGCTGCATCCAATTTGCGAGGTGGGGTCCCATCAAGATTCAGTGGCAGC48990.17116640875824168No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC48930.1709567744548023No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT48250.16858091901582284No Hit
GTTACACCCACTCGCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATC48160.1682664675606638No Hit
GCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC47970.16760262559977246No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG47910.16739299129633312No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC47330.16536652636308594No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC46970.1641087205424497No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT46690.16313042712639936No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA46090.1610340840920057No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC45930.16047505928283406No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA45520.15904255820933172No Hit
GGTTTCTGCTGATACCAATTTAAATAAGTAGCAATGCTCTCACTTGCCCG45230.15802932574270814No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG44940.1570160932760845No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT44930.15698115422551132No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG44850.1567016418209255No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG44560.1556884093543019No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG44480.15540889694971607No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA44280.1547101159382515No Hit
AGTGAGAGCATTGCTACTTATTTAAATTGGTATCAGCAGAAACCAGGGGA43990.15369688347162794No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG43730.152788468156724No Hit
GTCTGCATCTATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGA43500.15198486999353977No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT42650.1490150506948154No Hit
AACCAGGGGAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAATTTGCGA41800.1460452313960911No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT41180.14387901026055097No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC41060.14345974165367226No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA40910.14293565589507384No Hit
ACTGTCAACAGAGTTACACCCACTCGCCGTGGACGTTCGGCCAAGGGACC40730.14230675298475573No Hit
GCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCCACGG40720.1422718139341825No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTG40600.1418525453273038No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT40490.1414682157709983No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA40290.14076943475953374No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGCGGATCTGCGACTGATTTCACT40170.140350166152655No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC39720.13877790887685976No Hit
CCCCTGGTTTCTGCTGATACCAATTTAAATAAGTAGCAATGCTCTCACTT39430.13776467641023615No Hit
TCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGT39360.13752010305622356No Hit
CCCTGGTCCCTTGGCCGAACGTCCACGGCGAGTGGGTGTAACTCTGTTGA39100.13661168774131965No Hit
CACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCAATAGATGCAGACAGG38570.1347599180609386No Hit
GTGATGGTGACTCTGTCTCCAATAGATGCAGACAGGGAGGATGGAGACTG37680.13165034255992136No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC37540.13116119585189615No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC37400.13067204914387098No Hit
CCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACTTGCCG37310.13035759768871194No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG37250.13014796338527257No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA37140.12976363382896705No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37030.12937930427266156No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG36610.127911864148586No Hit
GAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTGCTACTTATTTAAAT36510.12756247364285372No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT36250.12665405832794982No Hit
GATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTCGGAGCCAGAG36080.12606009446820493No Hit
GGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTCGGAGCCAGA35690.12469747149584907No Hit
GACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA35630.1244878371924097No Hit
CACCATCACTTGCCGGGCAAGTGAGAGCATTGCTACTTATTTAAATTGGT35610.12441795909126324No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT35560.12424326383839711No Hit
GAGTAGCAGGAGCCCCAGGAGCTGAGCGGGGACCCTCATGTCCATGCTGT35410.1237191780797987No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG35350.12350954377635932No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA35020.12235655510744282No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCAT34830.12169271314655149No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC34780.12151801789368535No Hit
CTATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTGCT34770.12148307884311213No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT34210.11952649201101138No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG34180.11942167485929171No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG33380.11662655081343351No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT33000.11529886689165085No Hit
GTAGCAATGCTCTCACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCAAT32460.11341215816069658No Hit
CTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC32300.11285313335152494No Hit
CCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTC31860.11131581512630291No Hit
TTACTACTGTCAACAGAGTTACACCCACTCGCCGTGGACGTTCGGCCAAG31710.11079172936770451No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATAC31540.11019776550795964No Hit
TTTCCACCCTGGTCCCTTGGCCGAACGTCCACGGCGAGTGGGTGTAACTC31400.10970861879993446No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG31350.10953392354706831No Hit
CCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGC31320.10942910639534864No Hit
GACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCT30990.10827611772643213No Hit
GGACCCCACCTCGCAAATTGGATGCAGCATAGATCAGGAGCTTAGGGGCT30910.1079966053218463No Hit
CTCCAATAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCA30780.10754239766439436No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT30440.1063544699449046No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAC29690.10373404115191255No Hit
CGCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTG29520.10314007729216768No Hit
GAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGC29420.10279068678643541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)