FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149871
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC36180.31464399049980385No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT34910.30359927330978864No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA32350.28133590637558475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC31910.27750939018376847No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGGGCACTC30480.2650732125603654No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30370.2641165835124114No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC28720.24976714779310027No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28530.2481147885284523No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27140.23602647601339627No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26600.23133029705071267No Hit
GGATAGAGGCCTGAGTGCCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGA26190.22776467969015654No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC26150.2274168145818096No Hit
CCCAAGGGGCACTCAGGCCTCTATCCCATGTCCCGCAATAATAATCGGCC24060.20924086267068218No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT24050.20915389639359544No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23520.20454468370799855No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23430.20376198721421793No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22030.19158670842207517No Hit
ACATGGGATAGAGGCCTGAGTGCCCCTTGGGTGTTCGGCGGAGGGACCAA21610.18793412478443236No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21370.18584693413435072No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21270.18497727136348338No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT21140.18384670976135584No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21100.1834988446530089No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA20980.18245524932796808No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG20790.18080289006332015No Hit
GTTCCTGGGAGCTGCTGGTACCACGACACATAATTAGTCCCAATGTTGGA20650.17958536218410587No Hit
GAACATCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGACTAATT20620.1793244633528457No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATGAGCGACCCT20510.17836783430489161No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA20490.17819390175071811No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA20410.17749817153402425No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19980.1737586216192947No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19870.1728019925713406No Hit
GGACAGAACATCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAC19300.16784491477739677No Hit
ATCCCATGTCCCGCAATAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG19230.16723615083778962No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC18710.16271390442927947No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA18210.15836559057494276No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT17860.15532177087690704No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17510.15227795117887138No Hit
CCTCTATCCCATGTCCCGCAATAATAATCGGCCTCGTCCCCAGTCTGGAG17110.14879930009540202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16940.14732087338492753No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT16720.14540761528901935No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC16300.14175503165137657No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16280.14158109909720307No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAG16130.14027660494090208No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT16020.139319975892948No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15900.13827638056790717No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGGGCACTCA15850.13784154918247352No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC15700.1365370550261725No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.13636312247199903No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15520.13497166203861127No Hit
CCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC15170.13192784234057559No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGT14920.12975368541340723No Hit
ACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCGG14730.1281013261487593No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCACGACACATAATTAGTC14190.12340514718607565No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAA14050.12218761930686138No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTATTGCGGGACATGGGATAGAG13900.12088312515056038No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13700.11914379960882568No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13490.11731750779000429No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG13440.1168826764045706No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTATTGC13420.11670874385039712No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA13390.11644784501913692No Hit
GAATATCTCTGATCCCACCTCACCATTGAGAACCAGATTTGTGTACCATT13360.11618694618787673No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA13190.11470851947740224No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC13180.11462155320031552No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA13010.11314312648984103No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC12930.11244739627314715No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12510.10879481263550432No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG12490.10862088008133086No Hit
GATGAAGACAGTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTG12440.10818604869589718No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCCAGACTTGGAGCC12380.10766425103337679No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCA12190.10601189176872884No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11970.1040986336728207No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11850.10305503834777989No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11790.10253324068525947No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT11740.10209840929982582No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCA11730.10201144302273907No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA11720.10192447674565233No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG11650.1013157128060452No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGACTAATTATGTGTCGTGGT11650.1013157128060452No Hit
GCCCCCAAACTCCTCATTTATGACAATAATGAGCGACCCTCAGGGATTCC11600.10088088142061152No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTATTGCGGGA11520.10018515120391765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5700.060.4018172
GTGGTAT5750.057.6639481
CAACCGG354.8242047E-448.5771186
GGTATCA12250.041.9881671
TATATCG450.001662089637.7822075
TATCAAC30400.036.9122162
TTATACG709.240792E-636.432844
GTATCAA30500.035.6794131
ATCAACG31900.035.309783
TCAACGC31950.035.2545244
TCGAGGT3700.034.4575049
CAACGCA33300.033.8252835
AACGCAG35050.032.1364336
GTCGAGG3850.032.011178
ATCGCTC802.3080962E-531.8731888
ATAGGAC550.0044454630.9127123
ATATCGC550.0044454630.9127126
TATACGT550.0044454630.9127125
CGCAGAG38050.029.5975328
ACGCAGA38350.029.3672777
Produced by FastQC (version 0.11.5)