FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149871
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53190.4625736278243386No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT34860.303164441924355No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG34600.3009033187200999No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAAC30490.2651601788374522No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG28410.24707119320341153No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGGGCACTCA28330.24637546298671764No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCAC28220.24541883393876357No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTG27880.24246198051781462No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGGGCACTCAGGCCTCTATC26360.22924310640063103No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA23230.20202266167248326No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA23020.20019636985366185No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23020.20019636985366185No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22950.19958760591405472No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCT22770.1980222129264935No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAC22540.19602198855349862No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCC20970.18236828305088135No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20860.18141165400292728No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCG20160.1753240146068559No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGC20100.17480221694433548No Hit
GGATAGAGGCCTGAGTGCCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20090.17471525066724877No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCT18940.16471412880227435No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCT18510.16097457888754477No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC17880.15549570343108055No Hit
ACATGGGATAGAGGCCTGAGTGCCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC17780.1546260406602132No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCT17490.15210401862469788No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAACATCACCATCTCCTGCT17470.15193008607052444No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA16840.1464512106140602No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT16820.14627727805988672No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGG16490.1434073909160245No Hit
CCCAAGGGGCACTCAGGCCTCTATCCCATGTCCCGCAATAATAATCGGCCTCGTCCCCAGT16300.14175503165137657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCC16300.14175503165137657No Hit
GAACATCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGACTAATTATGTGTCGTGG16260.14140716654302962No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGAC15740.13688492013451944No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTG15680.13636312247199903No Hit
GGACAGAACATCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGACTAATTATGTGT15580.1354934597011317No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATGGAT15480.13462379693026436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTG15470.13453683065317762No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGA15360.13358020160522355No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAG15190.13210177489474906No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCG14450.12566627039033074No Hit
ATCCCATGTCCCGCAATAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGG14320.1245357087882032No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG13840.12036132748803996No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13790.1199264961026063No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTA13710.11923076588591243No Hit
CCCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC13680.11896986705465223No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTC13600.11827413683795836No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGAC13430.11679571012748387No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13330.11592604735661653No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGC13300.11566514852535634No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGGGCACTCAGGCCTCTATCC13240.11514335086283592No Hit
CCTCTATCCCATGTCCCGCAATAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC13080.11375189042944817No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCT12840.11166469977936656No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG12680.11027323934597881No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATC12550.10914267774385127No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCT12490.10862088008133086No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATGAGCGACCCTCAGGGATTCCT12390.10775121731046353No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCCAGACTTGGAGCCAGAGAATCGGT12200.10609885804581558No Hit
GTTCCTGGGAGCTGCTGGTACCACGACACATAATTAGTCCCAATGTTGGAGCTGCTTCCAG12030.10462043133534109No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC11960.10401166739573396No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGAGAATA11640.10122874652895845No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAA11600.10088088142061152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.043.183461
TGGTATC7000.039.285312
ACGTCTA350.004099840831.42824739
GGTATCA15150.028.1546211
GTATCAA36050.025.724991
TATCAAC35700.025.4199052
AGAGTAC23850.024.3299511
CAGAGTA24600.023.92355710
ATCAACG38950.023.2282583
GCGAGAG952.315317E-623.15866513
CCGTCGT600.00203513722.91742754
TCGCACA600.002036681922.91443620
TCAACGC39200.022.8666784
CAACGCA39900.022.6702755
AACGCAG41050.022.2361056
TGTGCGA752.8720137E-422.0007310
TGCCGAC1505.984475E-1022.0007355
GAGCGGA752.8745498E-421.9978617
CCTAGAT903.9959446E-521.3886683
AGATGCG650.003241578121.15178918
Produced by FastQC (version 0.11.5)