FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129630
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100540.4721007874607326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT93400.4385738367697675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC84910.39870775674647707No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC81620.3832590637810324No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT72070.33841559331902726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71700.3366782023168344No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT71200.33433037663819537No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA71110.33390776801604033No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG67380.3163929884533933No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT64020.3006155998929391No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG61500.28878255847259854No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC60470.2839460375746022No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC58420.2743199522921822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC57190.26854430112273026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG55320.25976343308462035No Hit
ATATAATAGTTATTCCCTCATGTACACTTTTGGCCAGGGGACCAAGCTGG54660.25666430318881683No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT53950.2533303907251494No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA52030.24431474011917562No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC52000.2441738705784573No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50650.23783474124613196No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT48380.22717561266511083No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA48350.22703474312439248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT47770.22431126533717125No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT45430.2133234411611407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC45380.21308865859327678No Hit
ACTTTAGAAAGTGGGGTCCCAGCAAGGTTTAGCGGCAGTGGATCTGGGAC44850.21059996337391942No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44220.20764170301883425No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGAGTATTAGTAG43420.20388518193301183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43160.20266431258011958No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC41740.19599648765278474No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG41390.19435300967773744No Hit
TTCTAAAGTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT39990.18777909777754823No Hit
CTAATACTCTGATTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA39470.18533735907176363No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA39150.18383475063743468No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC39110.18364692458314355No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG38060.17871649065800163No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT37260.1749599695721792No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG37070.17406779581429638No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAG36480.17129736151350233No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT36410.17096866591849288No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36100.1695130139977367No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG35530.16683649272408824No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC34540.16218779788038298No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT34500.16199997182609185No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC34200.16059127641890847No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA33880.1590886679845795No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA32340.15185736489437132No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA31850.14955649572930507No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31800.1493217131614412No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC31060.14584693115705544No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC31020.14565910510276434No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA30900.14509562693989098No Hit
GTCTCCTACAGATGCAGCCAGGGTGGAAGGAGACTGGGTCATCTGGATGT30780.14453214877701762No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGT30510.14326432291055252No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30500.14321736639697977No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA30160.14162084493550522No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC29870.1402591060418946No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT29840.14011823650117625No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAA29580.138897367148284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC29080.13654954146964496No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC28920.13579823725248047No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA28440.13354432460098703No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28410.13340345506026868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC28330.13302780295168645No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCA28300.1328869334109681No Hit
CAATATAATAGTTATTCCCTCATGTACACTTTTGGCCAGGGGACCAAGCT28260.13269910735667698No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA28140.13213562919380362No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT27940.13119649892234803No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG27840.1307269337866202No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA27720.13016345562374684No Hit
GAATATCTCTGATCCCACCTCACCATTGAGAACCAGATTTGTGTACCATT27710.13011649911017406No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.12861389067584508No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCA27340.1283791081079812No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG27290.12814432554011732No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC26950.12654780407864277No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG26450.12419997840000375No Hit
GATGAAGACAGTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTG26320.12358954372355761No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC26280.12340171766926648No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT26210.12307302207425702No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA26090.12250954391138368No Hit
CCTTCCACCCTGGCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG26070.1224156308842381No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCA25860.12142954409920972No Hit
CCTACTAATACTCTGATTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTA25580.12011476171917188No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25560.12002084869202631No Hit
GTTTAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC25520.11983302263773521No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC25510.11978606612416243No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT25380.11917563144771626No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25180.11823650117626067No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24930.11706258833694115No Hit
CTACAGAAGTGGAGCTGGATAATCAGATAGTTACTGCTACCCAGAGCAAT24800.11645215366049501No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGCTGGGACCCCA24530.11518432779402996No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24280.11401041495471044No Hit
CTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGT23720.11138085019463476No Hit
GGCCAAAAGTGTACATGAGGGAATAACTATTATATTGTTGGCAGTAATAA23620.11091128505890695No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA23610.11086432854533416No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC23370.10973737221958743No Hit
GTCTCCTTCCACCCTGGCTGCATCTGTAGGAGACAGAGTCACCATCACTT23250.10917389405671407No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC23140.10865737240741351No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23080.1083756333259768No Hit
CATTAGGATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACAC23010.10804693773096734No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA22940.10771824213595789No Hit
GACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTG22910.10757737259523956No Hit
TATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTG22770.10691998140522063No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22720.10668519883735672No Hit
CTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGTACACAAAT22640.10630954672877448No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG22470.10551128599803723No Hit
CTATAAGGCATCTACTTTAGAAAGTGGGGTCCCAGCAAGGTTTAGCGGCA22330.10485389480801828No Hit
GAGATATTCTCCCTGTTGTTCAGAGGAACAATAATTCGGATGTTTCTCTC22200.10424346013157215No Hit
TTATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTT22190.10419650361799937No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22010.10335128637368933No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC21430.10062780858646808No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21350.10025215647788584No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT21340.10020519996431305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)