FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129630
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160140.7519616083545029No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTG83220.39077210595267725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC78060.36654254494912264No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGA74420.3494503740086306No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG71550.3359738546132427No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACT70850.33268689866314805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTG69030.32414081319290206No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAG66050.31014777214821354No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGA64520.3029634255715782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACA63360.29751646999713566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG53790.252579086507985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCT53030.24901039147645368No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTG52220.24520691387705848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAG51540.2420138709541094No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGCTACACTTATGA51270.24074604508764436No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGA51090.2399008278433343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCAC50740.238257349868287No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCA49680.23327995942957228No Hit
ATATAATAGTTATTCCCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA49620.23299822034813558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC46030.21614083197550749No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT44940.21102257199607447No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG43670.20505909477233136No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCT42420.19918953057573383No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCA41690.19576170508492086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCC41580.19524518343562025No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC41400.19439996619131023No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCT41130.19313214032484516No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG40540.1903617060240511No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGG40480.19007996694261445No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC40360.1895164887797411No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGAGTATTAGTAGGTGGTTGGCCT38060.17871649065800163No Hit
ACTTTAGAAAGTGGGGTCCCAGCAAGGTTTAGCGGCAGTGGATCTGGGACAGAATTCACTC36380.17082779637777454No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGC35920.16866779675342666No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA35800.16810431859055328No Hit
TTCTAAAGTAGATGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATAC35440.1664138841019332No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGAGGGA35160.16509910172189535No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTG34270.16091997201391792No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGAT33580.15767997257739608No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGCTGGGACCCCACTTTCTAAAGT33070.1552851903851843No Hit
CTAATACTCTGATTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGCCAGGG33040.15514432084446594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACT32290.1516225823265074No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGA32050.1504956260007607No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCG31630.14852345243070392No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC31580.14828866986284003No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT30800.14462606180416315No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT30560.14349910547841643No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTG29920.1404938886097585No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGAC29750.13969562787902123No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCT29470.13838084549898338No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC29330.13772345430896446No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC29260.137394758713955No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC29260.137394758713955No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA28900.1357043242253349No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA28460.1336382376281326No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTA28050.1317130205716486No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG27750.1303043251644652No Hit
GTCTCCTACAGATGCAGCCAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATCTGGCA27370.12851997764869955No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCT27250.12795649948582616No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATGGAT27230.12786258645868062No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGT26760.12565563032075994No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACC26740.12556171729361437No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATC26540.12462258702215878No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTC26380.12387128280499429No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAA26010.12213389180280143No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGG25950.12185215272136475No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAA25070.11771997952696009No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCAGAGATATTCTC24980.11729737090480506No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGAGAATA24930.11706258833694115No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCA24810.11649911017406779No Hit
CAATATAATAGTTATTCCCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC24630.11565389292975777No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTG24430.11471476265830215No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGA24350.1143391105497199No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAATCAGATAGTTAC24310.11415128449542879No Hit
GATGAAGACAGTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTG24060.11297737165610927No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCC24010.11274258908824537No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACT23770.11161563276249865No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTAATACTCTGATT23460.11015998084174246No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAAC23370.10973737221958743No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAG23270.10926780708385964No Hit
GAATATCTCTGATCCCACCTCACCATTGAGAACCAGATTTGTGTACCATTTGTCTGACCTC22890.10748345956809399No Hit
CCTTCCACCCTGGCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGA22840.1072486770002301No Hit
CTACAGAAGTGGAGCTGGATAATCAGATAGTTACTGCTACCCAGAGCAATATCTGTGATGA22730.1067321553509295No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTG22690.10654432929663839No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22520.10574606856590112No Hit
GTCTCCTTCCACCCTGGCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAAT22420.10527650343017331No Hit
GTATCAACGCAGAGTACGGGAGAAGAAGTGAAGTCAAGATGAAGAACCATTTGCTTTTCTG22370.10504172086230942No Hit
CTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGTACACAAATCTGGTTCTCAA22320.10480693829444553No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT22240.10443128618586328No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTC22160.10405563407728104No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACA21840.10255302564295206No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC21820.1024591126158065No Hit
GAGATATTCTCCCTGTTGTTCAGAGGAACAATAATTCGGATGTTTCTCTCCACAATGTCCT21810.10241215610223371No Hit
GTCCCAGATCCACTGCCGCTAAACCTTGCTGGGACCCCACTTTCTAAAGTAGATGCCTTAT21550.10119128674934144No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG21370.10034606950503139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)