FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214355
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97994.571388584357725No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC10500.48984161787688646No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.2915723915933848No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA5580.26031583121457397No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC4080.1903384572321616No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAAT3600.16794569755778965No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.14228732709757178No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.13622262135242938No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.13249049474003405No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA2770.12922488395418816No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA2680.12502624151524339No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAA2550.118961535770101No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTT2460.11476289333115625No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA2400.11196379837185977No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA2330.10869818758601386No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATAC2300.10729864010636563No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC2260.10543257680016795No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATACCTGTCTCTTATACA2230.1040330293205197No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC2220.1035665134939703No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACA2160.10076741853467377No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.10076741853467377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA202.6052573E-455.0151861
CAGTTTC150.00577623855.00233522
CCCAGCG150.00577623855.00233542
ACGGCGA150.00577623855.00233516
GTTGCTT150.00577623855.0023358
GGTTGCT150.00578157954.9894947
AACGCAC150.005786923754.9766586
GGGTTGC150.005786923754.9766586
TTATCAA150.005786923754.9766582
CTTGGGT150.005786923754.9766583
CGTTGTG150.005786923754.9766584
CGCAATC257.8585383E-443.9813233
AGCGCCG1300.042.2897382
ATAAACG358.665757E-539.2690433
GTGGTAT11700.038.3225441
TGGTATC12000.037.3383142
CTGCGTA300.001921729236.66822410
ATCCTGC300.001923934436.6596647
TCGCAAT300.001926141836.6511042
GAGCGCC1550.035.4936681
Produced by FastQC (version 0.11.5)