FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478240
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT215971.460994155211603No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGG106890.7230896200887541No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT97230.6577416387054876No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88490.5986172745968178No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58130.39323790453512286No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGG45500.3077984630371252No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCT45190.3057013746076415No Hit
GAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAG43940.29724537287585234No Hit
GGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTT43390.29352473211386515No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGACTGTCTCT41810.28283634592488366No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC41720.28222751380019484No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG41380.2799274813291482No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG37140.2512447234549193No Hit
GAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAA36940.24989176317783307No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.2494182270808529No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC34970.2365651044485334No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.22810910271674423No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.223306093733088No Hit
GAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGG31410.21248241151639785No Hit
ATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC31370.2122118194609806No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGA30980.2095735469206624No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATCTGTCTCTTATAC29580.20010282498105855No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.1880614785149908No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG26490.17919958870007577No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT25670.17365245156402206No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA24470.1655346899015045No Hit
TATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGA23780.16086697694555688No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT23490.1589051845437818No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT21660.14652559800844248RNA PCR Primer, Index 46 (95% over 21bp)
CCTATTGACTATATTTTTTAAGTAGTTGTATTAATCTCTATCTTTCAAAG21550.14578146985604504No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.143616733412707No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC20420.13813724429050764No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCCTGTCTC19640.1328606992098712No Hit
GCAATATCTTAGTAACCTATTGACTATATTTTTTAAGTAGTTGTATTAAT18920.12799004221236066No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.12711061803225457No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGACTGTCTC18710.12656943392142006No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATCTGTCTCTTATA18370.1242694014503734No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATCTGT18190.12305173720099577No Hit
ATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA18110.12251055309016128No Hit
ATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGT18010.12183407295161815No Hit
AGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACC17840.12068405671609482No Hit
GAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGA17760.12014287260526031No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.12014287260526031No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT16830.11385160731680918No Hit
ATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAA16350.11060450265180215No Hit
GTATGTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCC16260.10999567052711332No Hit
CATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTT16170.10938683840242451No Hit
GCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTC16160.10931919038857019No Hit
TCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATA16120.10904859833315295No Hit
GTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCC15940.1078309340837753No Hit
ACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGG15830.10708680593137784No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.10485442147418551No Hit
AAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAA15330.1037044052386622No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT15140.10241909297543023No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.10126907673990691No Hit
CTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCTTAT14840.10038965255980084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC35000.066.662292
GTGGTAT35100.066.115851
CTTACGC452.9276262E-547.2208142
GGTATCA79550.043.5983471
GTATCAA162650.041.888951
ATATCAA6300.041.8283921
TATCAAC184400.038.3502662
ATCAACG203150.034.8536953
TCAACGC205450.034.463514
CAACGCA208100.034.0654875
AACGCAG214450.033.0964246
CAGGTAT5450.031.1948951
GTCGAGG27250.031.1906748
CTGAACG1800.030.692498
ACGCAGA238650.029.6670827
CGCAGAG238450.029.656328
GCAGAGT241900.029.2333589
TCGAGGT26750.029.2317839
AGGTATC4700.028.9353082
GCTTACG600.00680382528.3353631
Produced by FastQC (version 0.11.5)