FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478240
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1062847.189901504491829No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGA62060.41982357397986797No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGACTGTCTCTTATACACATCT43840.2965688927373092No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT38780.26233899772702673No Hit
GGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG38650.2614595735469207No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACGTTAAAAACTTA34200.23135620738175128No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTCAAGCTCCGCCT33470.22641790237038642No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATCTGTCTCTTATACACATCTGACGC31040.20997943500378827No Hit
GAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGATAAAT29480.19942634484251542No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT28480.19266154345708408No Hit
ATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC27310.18474672583612947No Hit
GAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT25740.17412598766100226No Hit
GAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGA24720.16722589024786233No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAG23550.1593110726269077No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAA22360.1512609589782444No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGACTGTCTCTTATACACATC19070.12900476242017533No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATCTGTCTCTTATACACATCTGACG17860.12081935274380344No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATCTGTCTCTTATACAC17460.11811343218963093No Hit
ATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG17020.11513691958004112No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC16960.11473103149691524No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG16580.11216040697045135No Hit
CCTATTGACTATATTTTTTAAGTAGTTGTATTAATCTCTATCTTTCAAAGAGAAAAAAACT16560.11202511094274273No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT16410.11101039073492802No Hit
ATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC16150.10925154237471588No Hit
GCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA15520.10498971750189413No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAA14970.10126907673990691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)