FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814455
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348000.7228232479065647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT46900.037.9702871
TGGTATC47750.036.7511372
GTATCAA155800.025.8634281
TATCAAC174800.023.052592
GGTATCA83400.022.7386991
AGAGTAC121000.022.20526711
ATCAACG181950.022.0374493
TCAACGC184500.021.7141234
CAGAGTA126600.021.48393810
CAACGCA187350.021.3964825
AACGCAG193150.020.8387366
GCGAACT4950.019.44305628
AGTACGG132450.019.39285313
ACGCAGA212300.018.8823077
GAGTACG137900.018.72613312
CGCAGAG217400.018.453728
TGCCGAC13300.017.57612255
GCAGAGT228200.017.5588179
CGAACTA5650.017.52087229
GTACGGG149500.016.79451214
Produced by FastQC (version 0.11.5)