FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820340
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213480.7569300155300425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29500.039.7293662
GTGGTAT31050.038.3821
GTATCAA100900.024.3045651
GGTATCA55450.023.5276241
TGCCGAC8400.023.24439255
AGAGTAC74000.021.92561311
TATCAAC112650.021.6140482
CAGAGTA79300.020.70370110
TCAACGC118800.020.5544034
ATCAACG118750.020.5431793
CAACGCA120700.020.2286915
AACGCAG124050.019.7045766
AGTACGG87500.018.54280313
TAACGGC2101.07320375E-1018.33458136
GAGTACG89850.017.99660912
ACGCAGA136250.017.940847
CGCAGAG136100.017.8403438
TACGGGG35050.017.1043915
GCAGAGT145000.016.823269
GCGTAAT1506.4128E-616.49995251
Produced by FastQC (version 0.11.5)