FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610440
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT39630.10976501479044104No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38680.10713375655044814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26150.037.2222251
GTATCAA74500.036.4571841
GGTATCA42250.034.4062961
TGGTATC28400.033.821942
ATCAACG84200.032.4064453
TCAACGC84600.032.3030174
CAACGCA85550.031.9939845
TATCAAC86500.031.6921772
AACGCAG88650.030.9231266
ACGCAGA99050.027.7594037
CGCAGAG99800.027.3800838
GCAGAGT115350.023.8360889
CAGAGTA74100.020.56038910-11
AGAGTAC66550.019.859710-11
GAGTACG77050.019.55311612-13
GTACGAC3301.364242E-1018.0311153
AGTACGG74350.017.46234912-13
GTACGGG87850.017.05268314-15
CTATACT11000.017.0005284
GTCGAGG12800.016.9322458
Produced by FastQC (version 0.11.5)