FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610440
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218850.6061588061288928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28650.033.9062651
TGGTATC30100.031.4387512
GTATCAA86150.024.5960451
GGTATCA47400.023.6870861
AGAGTAC68000.022.08160611
TATCAAC95800.021.9956362
TGCCGAC10600.021.53312755
TCAACGC99850.021.039924
ATCAACG99900.020.9502963
CAACGCA101150.020.877675
CAGAGTA72850.020.7247710
AACGCAG105500.020.0412376
AGTACGG78500.018.9528513
GAGTACG80150.018.66561312
ACGCAGA116050.018.1726787
CGCAGAG116200.018.1278178
CGTATAT1152.6201445E-416.7406063
GCAGAGT126450.016.70289
TATACCG2152.8921932E-916.6261485
TACGGGG43200.016.35890215
Produced by FastQC (version 0.11.5)