FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230220
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT47230.14621295143984No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.1438601705147018No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.11575062998805034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25400.049.697562
GTGGTAT26850.047.335431
GGTATCA50750.036.6857571
GTATCAA93400.036.4539871
TATCAAC102350.033.262662
ATCAACG104650.032.5717243
TCAACGC105950.032.131464
CAACGCA106400.032.035515
AACGCAG110400.030.9517976
ACGCAGA117950.028.9674287
CGCAGAG121000.028.20178
GCAGAGT136250.025.2007379
CAGAGTA82400.022.66627510-11
AGAGTAC75650.020.4756210-11
TCTAACG1254.7842576E-420.4010982
GTATAGA8450.019.6185681
GAGTACG95100.019.43870412-13
CCTATAC7100.018.557053
GTACGGG105850.017.24398414-15
AGTACGG89950.017.12637312-13
Produced by FastQC (version 0.11.5)