FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGAATGCTGTCATGAGATCGTTTGGCTTATGACTTTCATATTCCTATA11.2658227848101267No Hit
AAGATAAAAACCTTAATTTAAGTTCCAAAACAAAAAAAAAAAAAAAAACA11.2658227848101267No Hit
AAGTCCCGCAACGAGCGCAACCCTTTTCCTTATTTGCCAGCACTTCGGGT11.2658227848101267No Hit
GTCAAGACCACTGACTTGCTAAGCCCAACCCACTTTTTTATCTCCCTGTA11.2658227848101267No Hit
CAGCGTCCGATGTGTATAAGAGACAGACACAGTGTTTTGAGACCCACTGT11.2658227848101267No Hit
ACCTGAGAGATCAGGTGGTGCACCCCCCACCAACAGTCTTGGGCCAATGT11.2658227848101267No Hit
TGTCAATGATGAAAAAAAAAAAAAAAAAAAAAAAATAGGTTATATCAGTT11.2658227848101267No Hit
CCTACAACTTTTAAACTAAACTTTAAGCTGTTTAAGTCACCTTCATTTTA11.2658227848101267No Hit
CTTTGCTTTCTCTTGCTAAGGGTGACCGATCTGAGGACTTTGGTGTGAAT11.2658227848101267No Hit
AACCAAAATAGTATGCAAATAAAATCAAATCCTAAAAAATACAAAAAAAA11.2658227848101267No Hit
CAAAATCTTTTAACAACACACATAAATCCTGAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GACAGGAAGTATGGCGTGGGGACTTGTGGAACCAGAGGATTTTATGGCAC11.2658227848101267No Hit
GAATAGCACACGACACTTTGTGCTGATGCTGGATAAACCTGAAGTGTCAT11.2658227848101267No Hit
CTTATGGAGCATGTCATCAACACATTCTTCTCACAACATAGCAAGCGAAT11.2658227848101267No Hit
GAATGGAATCATCCGGAATGGAACAGAATGGAATGGAATGGAATGGAATG11.2658227848101267No Hit
CAAATAATGAACAAAACCTTAAACACAAAATCGGGGGTAGACAACCATCG11.2658227848101267No Hit
AAACTGAAGAAGACAGAGACGCAAGAGAAAACTACAATGACTTACAAAGA11.2658227848101267No Hit
GAGCTTCCTACAGAAAATTTACAAAATCTAGAAAATGATCTCAGTATACT11.2658227848101267No Hit
ACCCAGAGTAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGT11.2658227848101267No Hit
CCCTAGAAAAATGCCCACCTGCCACCAACATGAGAAGTCATCACACATGT11.2658227848101267No Hit
GTATCAACGCAGAGTACGGGGTGTGTTGATCTCTATGGTTAACGGTACAC11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGGCGT11.2658227848101267No Hit
AAATAAAATAATAAAAATAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GAATTGCTCTTGATTTTAGAAACAGCAACAGAAAAATGACCAAAAAGAAA11.2658227848101267No Hit
AAATAGTACAGGAGGGCAATTTGGAAGTATTATTAAAAACCAATATATAA11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11.2658227848101267No Hit
AATCAATAATAAGCAAAATAACATGGCAAGCACAAAGAAAGCTTCTGTAC11.2658227848101267No Hit
TGGTACAGCCTTCAGCCGGGGCTTCGGGAGACAGTGCAGCCACCGAGCAT11.2658227848101267No Hit
CACACACACACACACAACTTACACCAATCGAAATGAAAACAAAATAAATA11.2658227848101267No Hit
GTATTATCAAAGCAAAAAAATGGAACAACAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
ACCCTACACCCCTCCAGGCACATCTAACTCTGTCTCTTATACACATCTCC11.2658227848101267No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2658227848101267No Hit
AATGAAGTTTGAATAAGGCCTTCATCCATTTGATTTGCCATTAAGAAACT11.2658227848101267No Hit
GGCGAACGTCATGAGCATAGTGATCCCATTGGCGGCTGTCTCATAAAAAC11.2658227848101267No Hit
GCAGGCATCCTTGAGCTGTGGTGGGCTCCACCCAGTTCGAGCTTCCCGGC11.2658227848101267No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.2658227848101267No Hit
TACTATATATGGTTTTTTATTTTAAAATATTATTGGCCAGGTGCAGTGGC11.2658227848101267No Hit
AGCTTATCAAGAAGCCCTATAACTTTTCAATTTGAGATTCCTCACTCTTA11.2658227848101267No Hit
AGGATGGACTGGGACCCCAGCCTTTCCTGCCCCCTTTCAGGATCCCAAAG11.2658227848101267No Hit
GAAAAAACTAAGATACTACTATTTATAATATATTTTATTTTAATAATAAA11.2658227848101267No Hit
TTGCAGCAAGAATCGTATCGTGGCTCACAGCAAAACTAATAAAGTTGTTT11.2658227848101267No Hit
CCCTTATGCTACCCGGCAATGAGATCCATTTTCAAAACATATATAATTTA11.2658227848101267No Hit
GAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGC11.2658227848101267No Hit
GTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTCTACCCCCTA11.2658227848101267No Hit
CTTATTATAAATAAAAGTAACAAAATAAATTTAAATAATGACAAATTAAA11.2658227848101267No Hit
ACATTAATGTTATAGTTAGATTAATCAAAGAACAAGTAAAAATAATTTAT11.2658227848101267No Hit
TGCCAAAGCCATATCCTTTGTTGAAGACATCCTTAGCAGCCTTTCCTAGG11.2658227848101267No Hit
GGTAAAGAAATAATGGGATAAGATTACTAAACCCCACTATGTGCTTAAGA11.2658227848101267No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTAGGTGTATTTT11.2658227848101267No Hit
GGTTTGTTAGGTACTGTTTGCATTATTAAATTAAAGCTCCATTGGGTCTT11.2658227848101267No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCGGGGATGCCGTCGTCAGGT11.2658227848101267No Hit
ATTTTTAATGATCTCGGATGACCAAACCAGCCTTCGGAGCGTTCTCTGTC11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACAC11.2658227848101267No Hit
CTTATACACATCTCCGCGCCCACGAGACTACGCTGCATCGGGTGTTGCGG11.2658227848101267No Hit
TCTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATATCGTATGCCG11.2658227848101267No Hit
GAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTGGTTT11.2658227848101267No Hit
AAATATAAATTTATTAATGTGAAAATAAATTAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
TCCAGGTCCTCGTCTTCATGCTGCTGAGGAGCTGGGGTGACCAGCATTGG11.2658227848101267No Hit
CACTTACACCGGGCCCTCCAGCTCCTAGACGAACTACTTCATACCATGCC11.2658227848101267No Hit
ATACAAATCAAACACCAAAACAGACTTCATCTAAAATATAAAAACATCAA11.2658227848101267No Hit
GGTGCGTCGCTCGCATAATCGGGCACATCGTAAGGACTGTCTCTTATACA11.2658227848101267No Hit
AAACAGCTTAAAGTTTAGTTTAAAATTTGTAGTTGATTAAAATAATTTGA11.2658227848101267No Hit
GAACAACATAGTGAGACTGTCTCTCAAAAAGTAAAAAATTAGCGGGTATG11.2658227848101267No Hit
CTGCACTCCAGCCTGGGCGACACAGCGAGACTTCGTCTCAAAAAAAAAAA11.2658227848101267No Hit
GTATAGGGTGGAGCCTGAGGAAGATGCAAGGTCTGAGGGTATACTGGATT11.2658227848101267No Hit
GTGATAAAAGAAGATTACACTAAAGGATAAGGTAATAGTTGAACAACTAA11.2658227848101267No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG11.2658227848101267No Hit
CTTCATGGCAGCACGAAGTTCATCAGCATAAAATTTCGCTGTAGTTTTTA11.2658227848101267No Hit
AAGAATATTAGAGTATTTTTTTTATATTTTTTTTTATATATATAAAGGTC11.2658227848101267No Hit
TATTTAGCAGAGTATATCTGAGAATATATGGCCACTTATATTCTAAAAAT11.2658227848101267No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCCAATCGCGTAAGCCCT11.2658227848101267No Hit
GTACTACACTGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGTG11.2658227848101267No Hit
GCAGCAGCCTGCTCTTCTTTTTCAATCTCTTCAGGATCTCTGTAGAAGTA11.2658227848101267No Hit
CTTTTCCTTCCTCATGCTAGTCTTCTAAGTCTGCATATTCTGTATCTTAT11.2658227848101267No Hit
CACATAGATGATCCTGGAGCACATATCATATGTATATGCAGGTGTTTCTC11.2658227848101267No Hit
CAATGCCATATATGACATGGTAATCACACAAATACATTTAAGACTTAACC11.2658227848101267No Hit
CTCCTGTTTCCTCAAATGCTTCATTATGGCCTACTCCCCTTTTCCTCCCC11.2658227848101267No Hit
GGACAGAAAACAGGTTAGCGGTTGCCAAGGAGTGGGAGAAGCAGCCAGGG11.2658227848101267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGA50.085.08
CCTACAG50.085.07
AGCTTCC50.085.02
TACAGAA50.085.09
TCCTACA50.085.06
GAGCTTC50.085.01
TTCCTAC50.085.05
GCTTCCT50.085.03
CTTCCTA50.085.04
Produced by FastQC (version 0.11.5)