FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306500
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118540.9073096058170684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14250.038.0419921
TGGTATC15200.035.6479722
GTATCAA50200.028.230291
AGAGTAC36450.025.12709411
TATCAAC57100.024.807522
CAGAGTA38550.023.97234310
ATCAACG61150.022.9810643
TCAACGC61250.022.8977684
CAACGCA61400.022.8813615
AACGCAG61700.022.8146656
GGTATCA25350.021.8053863
AGTACGG42500.021.61489513
GAGTACG44350.020.77527412
ACGCAGA67500.020.691377
CGCAGAG68550.020.4176678
ATTCCGT956.0715225E-520.2583356
TGCCGAC4400.019.99906755
ACGGGAT7300.019.96405616
GCAGAGT73050.019.2019589
CGGGATT3800.018.8141917
Produced by FastQC (version 0.11.5)