FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732194
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67010.9151946068938014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10300.040.910391
TGGTATC10400.039.9491232
GTATCAA36900.026.7199761
TATCAAC41050.023.9957052
GGTATCA18400.023.6493231
CGAATTA952.3143748E-623.15754915
AGAGTAC28900.023.02576611
CGAATCG600.002035003922.91632516
CAGAGTA29850.022.6614310
ATCAACG44400.022.1111583
TCAACGC44800.021.9107444
CAACGCA45300.021.4838755
CGTCGGG650.003210790721.1853711
ACTATCG650.003240203521.15208611
AACGCAG46100.021.1110536
CGGGATC2750.020.99968717
GCTTGTA1902.5465852E-1120.2933561
TATCCGG700.00497476819.64256519
GATACCG856.6936994E-419.4061745
CGCAGAG50800.019.1050538
Produced by FastQC (version 0.11.5)