FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503058
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.3659617777671759No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16520.32839155723594493No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.3085131336744471No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.22124685423947138No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.14948574518246405No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.1266255580867415No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.12105959948952208No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC5840.11608999359914761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8200.061.167692
GTGGTAT8450.059.3579981
GTCGACA250.007795233350.9974948
GTCTAAG951.1405064E-940.2691841
GTATCAA31150.039.299621
GGTATCA17500.034.2479861
TATCAAC37000.033.6604422
GTCGAGG4300.032.6146778
AACGCAG39800.031.3991726
CAACGCA40050.031.2031755
TCAACGC40050.031.2031754
ATCAACG40200.031.0867423
CTGAGTC550.004446722630.9075748
CGCTATA703.9366624E-430.3616892
ACGCAGA44150.028.20647
CGCAGAG44150.027.9148248
GCAGAGT46400.026.5611959
AGGCCCG1159.150395E-625.8734366
GGCACCG1701.1883458E-824.9987749
CAGAGTA26950.024.28452110-11
Produced by FastQC (version 0.11.5)