FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503058
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50461.003065252913183No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTCC202.6135877E-454.99413718
TGGTATC9600.044.427222
GTGGTAT10150.042.3119771
CTCGCAC453.7927003E-430.55533428
GGCCGCG1450.028.44806940
TATTAGA2150.028.1561262
CGGTAGA705.650594E-627.529911
GGTACTA400.00786927127.49980221
AGTTTCG606.311125E-527.49980234
CTACTCT507.0266804E-427.4998024
AACGGCC1500.027.49980237
TGCGAGA400.00786927127.49980226
CCTTCGC1103.947207E-1027.49706820
CCGCCGC400.00787308927.49706814
GGCCCTA507.0307654E-427.49706612
GGTATCA21100.026.747071
CTGCCCA1450.026.54889317
GCTTGTA1256.002665E-1126.4287131
AGATCTC1057.239578E-926.19028742
GTATCAA40450.026.1346971
Produced by FastQC (version 0.11.5)