FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765982
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT19340.2524863508541976No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.17637490176009357No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.14334540498340692No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.10196062048455448No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC7810.10196062048455448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6400.047.15172
GTGGTAT6650.046.0242271
GGTATCA13700.037.233711
GTATCAA24700.037.1734161
TATCAAC28200.032.706162
ATCAACG28400.032.4758383
TCAACGC28650.032.192454
CAACGCA29200.031.5860865
GTCGTAC703.937413E-430.3632051
AACGCAG31100.029.929726
CGCAGAG33550.027.7422838
ACGCAGA34500.026.9783657
GCAGAGT37450.024.7381349
GTACGGA1051.4680097E-424.2905651
CAGAGTA22400.022.67159810-11
GTATAAC1353.1670334E-522.0414371
GAGTACG24200.020.80967112-13
ATACGGG1254.7749738E-420.4040761
GTCGAGG3750.020.4000788
AGAGTAC20550.020.36979710-11
Produced by FastQC (version 0.11.5)