FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765982
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60990.7962328096482684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8100.040.0758632
GTGGTAT8250.040.042891
GCCGTTA453.7937926E-430.55635515
GCCGACG904.7171852E-827.49892255
CTACACG957.967719E-826.051614
GTATCAA29450.024.6783851
GGTATCA15750.024.1210731
AGAGTAC20100.023.94497511
TGCCGAC2100.023.57050355
ACGGGTA852.570948E-522.64469116
CAGAGTA21350.022.54304310
TACGGGT2950.022.37346615
TATCAAC32350.022.1948432
TCAACGC33250.021.337514
CCGTTAA650.003240737421.15163616
ATCAACG33950.021.065063
CAACGCA33950.020.977195
TCTGTCG1055.5157216E-620.951568
ACGGGCT1055.518841E-620.95019116
GAATACT1207.588351E-720.6241934
Produced by FastQC (version 0.11.5)