FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687867
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT22690.32986027822238895No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.1910252999489727No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.14653995612523935No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.1216804992825648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6150.049.763892
GTGGTAT6900.044.354771
GGTATCA13100.041.208741
GTATCAA23400.036.148821
TATCAAC26850.031.1873762
TCAACGC27650.030.438764
ATCAACG27900.030.3183633
CAACGCA27850.030.220175
AACGCAG28950.029.2187336
TACACCG1054.5154557E-628.3377695
CGCAGAG31250.027.0623328
ACGCAGA31900.026.5109067
GCAGAGT34900.024.2320359
CAGAGTA19850.024.19246710-11
GTCCTAA2352.7284841E-1123.514321
TAGGACC2454.9112714E-1122.554554
AGAGTAC18200.022.182710-11
GTCGAGG4150.021.50478
GAGTACG21900.021.44274312-13
ACCGGGC1000.003155514621.2486928
Produced by FastQC (version 0.11.5)